2015-06-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/683967摘要：草蝦雖是全世界最重要的養殖蝦種，然近&#63886;&#63789;飽受疫病侵襲且野生族群&#63870;逐漸下降。學界對於對蝦類生長、生殖、免疫調控等方面&#63823;積之基礎生物學極少，對基因體之瞭解&#63745;是有限。本計畫以瞭解對蝦基因體為目標，擬由數方面著手：(一) 利用『RAD標記定序法』，對太平洋與印度洋草蝦族群進行次世代定序，尋找兩者間特有之SNPs分子標記以便進行分子育種。(二)利用生物資訊方式，由基因體序列資料庫中搜尋重要基因之基因體序列；並以實驗方式，獲取相鄰片段間之未知序列，以求得重要基因之完整定序，俾使抗病等分子研究得以獲得基因DNA marker。（三）設定適當的交配流程獲得F1子代，並利用『目標富集』定序法，尋找與抗病毒基因相關之SNPs，以利於培育抗病之對蝦品系。<br> Abstract: The black tiger shrimp (Penaeus monodon) is the most important shrimp aquaculture species in the world especially in the Indo-Pacific region. The shrimp industry is now threatened by diseases and depletion of the wild broodstock. While past research efforts have been on shrimp pathogens, the information about the host P. monodon remains scanty. Basic knowledge of shrimp biology, particularly with regard to the control of growth, reproduction and the immune system, is limited and hampered by the lack of genome information. To facilitate our understanding of the genome of Penaeid shrimps, we attempt to conduct the following studies : (1) To perform a genome scan of nucleotide diversity and differentiation in natural populations (Indian Ocean vs. Pacific Ocean) of Penaeus monodon by using Restriction-site associated DNA (RAD) sequencing to identify and type single nucleotide polymorphisms (SNPs). (2) To obtain full length of the genes implicated in WSSV resistance/ susceptibility through bioinformatic search against our in-house genomic sequence database, and to design primers to fill the gaps between adjacent contigs. (3) To design an appropriate breeding program producing F1 offspring with opposed phenotypes (“WSSV-resistant” vs. “WSSV-sensitive”), then apply target enrichment and NGS technique to identify the SNPs underlying the WSSV-resistance/susceptibility.草蝦基因體單核&#33527酸多型性分子育種RAD標記定序法目標富集定序法Penaeus monodontiger shrimpgenomeSNPsmarker-assisted selectionRADtarget enrichment