Hsieh, Y.-F.Y.-F.HsiehYonezawa, E.E.YonezawaKuo, L.-S.L.-S.KuoShiou-Hwei YehPEI-JER CHENPING-HEI CHEN2021-07-032021-07-0320130003-6951https://www.scopus.com/inward/record.uri?eid=2-s2.0-84983718347&doi=10.1063%2f1.4803442&partnerID=40&md5=73b9ebe9de5361efc809b92ebafad74ahttps://scholars.lib.ntu.edu.tw/handle/123456789/568454This research demonstrated that without any external temperature controller, the capillary convective polymerase chain reaction (ccPCR) powered by a candle can operate with the help of phase change. The candle ccPCR system productively amplified hepatitis B virus 122 base-pairs DNA fragment. The detection sensitivity can achieve at an initial DNA concentration to 5 copies per reaction. The results also show that the candle ccPCR system can operate functionally even the ambient temperature varies from 7 °C to 45 °C. These features imply that the candle ccPCR system can provide robust medical detection services. ? 2013 AIP Publishing LLC.[SDGs]SDG3Viruses; Detection sensitivity; DNA concentration; DNA fragment; External temperature; Hepatitis B virus; Phase Change; Thermal control; Polymerase chain reactionjournal article10.1063/1.48034422-s2.0-84983718347