李嘉哲2006-08-312018-07-112006-08-312018-07-112002http://ntur.lib.ntu.edu.tw//handle/246246/29777人類白蛋白是肝所製造的重要蛋白之 一，它在維持血液滲透壓扮演重要角色， 並作為各種荷爾蒙，蛋白質，脂肪，離子 及藥物的載體。我們發現白蛋白的表現， 會隨培養液中白蛋白的濃度的增加而減 少。這顯示肝細胞可能含有白蛋白受體。 我們先用噬菌體呈現法來尋找此受體，但 是效果不佳，因此改用了細菌二雜交系 統。我們找到了兩個可能的基因，分別為 phosphodiesterse 3B 及ATP-binding cassette member 4 。我們已選殖此基因，現正研究其功能中。Human serum albumin in one of major proteins that are produced by the liver. It plays an important role in maintaining intravascular oncotic pressure and acts as a carrier for various hormones, proteins, fatty acids, ions and drugs. We found that expression of albumin in hepatocyte or hepatocellular carcinoma cell line correlated inversely with the concentration of albumin in the culture medium. This implied that albumin receptor possibly existed in these cells. Initially, we used phage display system to find out this receptor, but failed. We switched to BacterioMatch two-hybrid system and revealed two candidate genes, phosphodiesterse 3B and ATP-binding cassette, member 4. We Have cloned these genes. We are currently defined their roles as possible receptor of albumin.application/pdf34059 bytesapplication/pdfzh-TW國立臺灣大學醫學院內科白蛋白受體細菌二雜交系統AlbuminReceptorBacterioMatch two-hybrid systemreporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/29777/1/902314B002219MH.pdf