Wu P.-C.Chen Y.-S.Hsieh T.-Y.Liu H.-W.Win-Li LinLiu T.-M.2020-02-262020-02-2620121605-7422https://scholars.lib.ntu.edu.tw/handle/123456789/464819The understanding of the interaction between tumors and surrounding microenvironment in vivo is an important first step and basis for pathway-targeting cancer therapy. To in vivo observe the dynamic development of tumor cells and validate the efficacy of therapy in microscopic scales, people commonly performed multi-photon fluorescence microscopy through an invasive window chamber setup. However, under such system, the cancer cells can't be identified and long-term tracked without a fluorescence labeling. Exploiting the intrinsic third harmonic generation (THG) and two-photon fluorescence (2PF) contrasts of melanin, we demonstrated in vivo identification of melanoma and tracked its development without labeling. It was achieved with a least invasive femtosecond Cr:forsterite laser and a laser scanning nonlinear microscopy system with 3D sub-micron spatial resolution. Combined with molecular probes or reporters, we anticipate thus developed platform a powerful tool to reveal molecular insights of tumor microenvironments, enhance our understanding of tumor biology, and trigger new therapeutic approaches. ? 2012 Copyright Society of Photo-Optical Instrumentation Engineers (SPIE).Cell tracking; harmonic generation microscopy; melanoma; tumor microenvironments; two-photon fluorescence[SDGs]SDG3Cell tracking; harmonic generation microscopy; melanoma; Microenvironments; two-photon fluorescence; Dermatology; Fluorescence; Fluorescence microscopy; Harmonic generation; Medical applications; Nanoparticles; Photons; Probes; Tumors; Oncologyconference paper10.1117/12.9066822-s2.0-84859604821