CHANG-YAO HSIEHCHI-AN CHENHuang C.-Y.Chang M.-C.CHIEN-NAN LEESu Y.-N.WEN-FANG CHENG2020-11-272020-11-2720071525-0016https://www.scopus.com/inward/record.uri?eid=2-s2.0-34548807539&doi=10.1038%2fsj.mt.6300243&partnerID=40&md5=396412b1c22f2ee8e8d8edfd05e45db9https://scholars.lib.ntu.edu.tw/handle/123456789/522671A naked DNA vaccine delivered by gene gun into antigen-presenting cells (APCs) has emerged as an attractive strategy for antigen-specific cancer immunotherapy. However, APCs have a limited lifespan, hindering their long-term ability to prime antigen-specific T cells. Furthermore, the potency of DNA vaccines is limited by their inability to process and present antigens. Interleukin-6 (IL-6) could play a role in immunity and cell apoptosis. We explored how the DNA vaccine encodes IL-6 to a model tumor antigen, human papilloma virus type-16 (HPV-16) E7. Mice vaccinated with IL-6/E7 DNA exhibited dramatic increases in E7-specific T-cell immunities, anti-E7 antibody responses, and impressive anti-tumor effects against E7-expressing tumors. The in vitro results revealed that IL-6 enhances DNA vaccine potency through the major histocompatibility complex class I pathway via direct and cross-priming effects. In addition, the delivery of IL-6/E7 DNA prolonged the survival of transduced dendritic cells (DCs) in vivo. Our results indicated that the IL-6/E7 DNA vaccine combined the mechanisms of enhancing antigen processing and presentation with prolonging the survival of DCs. Using IL-6 represents an innovative approach to enhancing DNA vaccine potency and holds promise for cancer prevention and immunotherapy.[SDGs]SDG3DNA vaccine; green fluorescent protein; interleukin 6; major histocompatibility antigen class 1; protein mcl 1; tumor antigen; virus vector; animal experiment; animal model; antibody response; antigen presentation; antigen presenting cell; antigen specificity; antineoplastic activity; article; cancer immunotherapy; cancer prevention; cell based gene therapy; cell survival; cellular immunity; controlled study; dendritic cell; drug potency; expression vector; female; genetic transduction; Human papillomavirus type 16; in vitro study; in vivo study; malignant neoplastic disease; mouse; nonhuman; viral gene delivery system; Animals; Antigens, Neoplasm; Apoptosis; Base Sequence; DNA Primers; Female; Humans; Immunity, Cellular; Interleukin-6; Mice; Mice, Inbred C57BL; Neoplasms; T-Lymphocytes, Cytotoxic; Vaccines, DNA; Human papillomavirus; Musjournal article10.1038/sj.mt.6300243176096582-s2.0-34548807539