2014-01-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/699433Abstract: Biological interactions are hardly ever unitary and typically involve a complex cascade of immobilizations and simultaneous manipulation of several physical and chemical properties on surface. Yet, almost all synthetic surface modification concepts rely on a single type of surface cues; achieving controlled, simultaneous presentation of two or more biomolecules remains challenging. Multifunctional coatings prepared via chemical vapor deposition (CVD) copolymerization process are exceptional candidates to overcome these challenge. These coatings can control surface properties independently from the bulk material(s) and not only expand the diversity of immobilization schemes, but also ensures that a proven immobilization method can be easily transferred from one substrate to another, without the need for continuous re-configuration of the underpinning substrate chemistries. We already showed that a monofunctional CVD coating can be used, regardless of the substrate materials, to immobilize functional compounds via “click-chemistry” by Dip-Pen Nanolithography (DPN) which is previously limited by the number of substrates/ink combinations. We would now like to expand that work to multifunctional coatings, enabling the simultaneously use of broadly available functional molecules on the same substrate. The precise control of multiple surface reactions, while avoiding cross-reactivity between the different chemical groups is key to overcome challenges to this field and will benefit applications such as the regulation of cell shape, the development of advanced biological assays and scaffolds for regenerative medicine, or the fabrication of increasingly complex micrototal analytical systems (µTAS) that all demand defined surface architectures and usually require precise immobilization of multiple biomolecules.