Lu F.-J.Lin J.-T.HSIU-PO WANGHuang W.-C.2021-01-152021-01-151996https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030046142&doi=10.1007%2fBF01923359&partnerID=40&md5=60d0bc90b14b2c6d2bd3aa1977782406https://scholars.lib.ntu.edu.tw/handle/123456789/541318A simple, sensitive, non-stimulated assay was developed to measure the superoxide anion concentration in whole blood, using an ultra-sensitive chemiluminescence (CL) analyzer and lucigenin amplification. The assay system can be performed without leukocyte isolation or stimulant administration. The blood CL levels of healthy males (362.8 ± 337.7 counts/10 sec) were not different from those of females (335 ± 308.7 counts/10 sec) (p = 0.64), whereas the CL levels in whole blood in patients with acute pancreatitis (2522 ± 2014 counts/10 sec) were significantly higher than those of healthy controls (p < 0.001). This assay system may be valuable in the future for quantitative measurement of reactive oxygen species in various disorders.Chemiluminescence; Free radical; Pancreatitis; Reactive oxygen species; Superoxide[SDGs]SDG3lucigenin; superoxide; acute pancreatitis; adult; aged; analyzer; article; assay; blood chemistry; chemoluminescence; clinical article; controlled study; female; human; male; photon; Acridines; Acute Disease; Adult; Aged; Aged, 80 and over; Biological Markers; Chemiluminescent Measurements; Female; Humans; Male; Middle Aged; Pancreatitis; Photons; Reactive Oxygen Species; Reference Values; Sensitivity and Specificity; Sex Characteristics; Sex Factors; Superoxidesjournal article10.1007/BF0192335986088152-s2.0-0030046142