李芝珊2006-07-252018-06-302006-07-252018-06-302005-07-31http://ntur.lib.ntu.edu.tw//handle/246246/4886雖然培養分析仍是環境生物氣膠的主 要分析方法， 但為了要更了解及定量生物 氣膠， 應該一起使用培養及非培養的方法 並加以比較。在此流式細胞儀及螢光染色 法用不同的螢光染劑染色（ 細胞膜的完整 性及代謝） 用於去評估衝擊（ AGI-30 all-glass impinger ） 及過濾 (track-etched polycarbonate filter) 的 採樣成效， 然後和傳統的培養方法做比 較。兩種細菌性氣膠和兩種真菌性氣膠被 作來研究。生物氣膠的特性（ 堅硬的或脆 弱的） 在採樣過程中高度影響著生物氣膠 的活性， 就如螢光染劑會有不同的物理機 制一樣。對於被採樣的生物氣膠活性方 面， 衝擊器比過濾器更好。此外， 發現在 過濾時採樣的壓力對生物氣膠代謝機制的 影響較細胞膜的完整性大。再者， 也發現 由於採樣壓力所造成不同的細胞膜完整性 及代謝性和生物氣膠的種類有關。Although culture-based analysis remains the primary method for environmental bioaerosol analysis, for better understanding and quantifying of bioaerosols, both culture and nonculture-based methods should be used and compared. Here, flow cytometry with fluorochrome (FCM/FL) was applied to evaluate the sampling performance of impingement (AGI-30 all-glass impinger) and filtration (track-etched polycarbonate filter) with different types of fluorescent dye staining (cell membrane integrity and metabolism) and then compared with a traditional culture method (culturability). Two bacterial aerosols (Escherichia coli and endospores of Bacillus subtilis) and two fungal aerosols (Candida famata and Penicillium citrinum spores) were studied. The bioaerosol viability during the sampling processes was highly influenced by bioaerosol characteristics (hardy or fragile), as well as by the fluorescent dyes with different physiological mechanisms. For better viability of the sampled bioaerosol, the impinger was superior to the filter. Moreover, it was found that sampling stress from filtration had more influence on the bioaerosol metabolism mechanism than cell membrane integrity. Furthermore, the differences between cell membrane integrity and the metabolism by sampling stress were found related to the bioaerosol species.application/pdf242248 bytesapplication/pdfzh-TW國立臺灣大學公共衛生學院環境衛生研究所生物氣膠採樣效率流式細胞 儀bioaerosolsampling efficiencyflowcytometryreporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/4886/1/932320B002050.pdf