2010-08-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/705454摘要：臺灣杉為臺灣原生之重要經濟樹種，屬新生代第三紀孑遺植物，為世界級之珍貴「活化石」樹種。針對臺灣杉的抽出成分、生物活性、及其木材性質的研究，已累積相當多的成果。萜類化合物為臺灣杉所含重要二次代謝物，此類化合物又與樹木之生理作用、授粉、防禦機制、逆境調節等方面有著密切的關係；同時，許多重要香料之主要成分亦為萜類化合物，又因為此類化合物常具有特殊的生物活性，其醫藥保健之功能已越來越受重視。因此，如能瞭解萜類化合物於樹木中生合成之機制，將有助於進一步瞭解萜類化合物於自然界中所扮演的角色，同時藉由萜類化合物生合成相關基因的序列分析，將可探索其於演化、分類地位上之親緣關係。為進一步於分子層面了解臺灣杉萜類化合物之生合成及在臺灣杉生長發育和防禦之調控，本計畫將進行臺灣杉萜類合成酶基因之選殖與功能分析，配合基因體學、轉錄體學、蛋白質體學、代謝體學、生物資訊學與基因轉殖等研究技術及策略，深入探討萜類化合物之生合成機制。此外，此研究成果亦可提供林木生理、林木改良、演化、保育及醫藥開發之重要參考資料。<br> Abstract: Taiwania (Taiwania cryptomerioides Hayata) is one of the most economically important native tree species grown in Taiwan. Besides, it is the “living fossil” from the Tertiary period of the Cenozoic era. Up to date, many of studies conducted with the utilization bioactive compounds of Taiwania. Terpenoids are one of the most important and abundant secondary metabolites in Taiwania, they also implicated in the attraction of both pollinators, protection against photo oxidative stress, mediating thermo tolerance, and direct defense against microbes and insects. Many of the terpenoids are commercially interesting because of their use as the flavors and fragrances in foods and cosmetics. In addition, some of terpenoids are used as raw materials for pharmaceuticals production. Moreover, it will be helpful for exploring the evolution and phylogenetic taxonomy by analyzing the gene sequences of terpenoid synthases. To further understand the terpenoid synthase genes of Taiwania related to growth, development and defense regulation, we will clone and characterization the terpenoid synthase genes in Taiwania. By using the genomics, transcriptomics, proteomics, metabolomics, bioinformatics and transgenic technology, the biosynthesis mechanism of terpenoids will be elucidated for Taiwania, and we believe that it will make a great contribution for understanding the function of terpenoids in Taiwania. Moreover, the results obtained in this project will provide the useful information for tree physiology, tree improvement, evolution, conservation and drug discovery in the future.臺灣杉基因萜類合成酶生合成Taiwania cryptomerioidesgeneterpenoid synthasebiosynthesis臺灣杉帖類化合物之生合成機制及其調控