2013-02-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/689026摘要：家禽流行性感冒病毒(Avian influenza virus, AIV)依致病力分為高病原性與低病原性；其中H5與H7亞型的低病原性病毒容易因突變而轉變為高病原性病毒。2012年已證實原本在台灣近十年所流傳的低病原性H5N2已突變為高病原性；顯示高、低病原性病毒的監測控制與鑑別診斷的重要性。馬立克病毒(Marek`s disease virus, MDV)、家禽白血病病毒(avian leukosis virus, ALV)與網狀內皮細胞增生病毒(avian reticuloendotheliosis virus, REV)，會造成禽類淋巴球增生性腫瘤性疾病，造成全球養雞產業上極大的衝擊。急性馬立克病會造成雞隻35%的高死亡率。在家禽白血病方面，1996至2002發生的ALV-J病毒引起全球經濟極大的損失。2010年台灣進口但用種雞雞群的家禽白血病抗體陽性盛行率為86.7%，肉用種雞抗體陽性盛行率為97.8%。家禽白血病病毒分為A、B、C、D、E、J六個亞群，ALV-A為常見的外源性病毒，ALV-E為內源性病毒，而ALV-J則是由外與內源性病毒基因重組所產生之強毒亞群。網狀內皮增生症在2003-2005年台灣種雞血清學的抗體陽性率為92.8%。此三種病毒所造成的病變相似，腫瘤沒有典型的病理區別診斷證據可供參考，造成診斷上極大的困難，必需要作進一步的鑑別診斷。本計畫首次嘗試以生物晶片來鑑別診斷高與低病原性家禽流行性感冒病毒；以及馬立克病毒、家禽反轉錄病毒(ALV-A, ALV-J, ALV-E)、網狀內皮細胞增生病毒所造成的腫瘤性疾病。希望利用生物晶片高敏感度與同步多工性，來達到快速篩檢與正確鑑別診斷的目的，促進這些疾病的控制以及家禽產業的競爭力。<br> Abstract: Avian influenza viruses (AIV) are classified into highly pathogenic and low pathogenicity based on their virulence. Low pathogenicity H5 and H7 subtypes of AIVs are easily mutated to become highly pathogenic. The prevailing low pathogenicity H5N2 in Taiwan in the last decade has been proven to become highly pathogenic in 2012, demonstrating the importance of virus surveillance and the differentiation diagnosis. Marek`s disease virus (MDV), avian leukosis virus (ALV), and avian reticuloendotheliosis virus (REV) cause avian lymphoproliferative neoplasms and bring about a big impact on poultry industry in the world. Acute Marek`s disease can cause a high mortality of 35% in chicken. The epidemics caused by avian leukosis virus subgroup J from 1996 to 2002 had incurred big global economic losses. The prevalence of serum antibody against ALV was 86.7% in imported egg-type breeding chicken and was 97.8% in imported broiler breeder flocks in Taiwan in 2010. ALVs are classified into A, B, C, D, E and J subgroups. ALV-A is the common exogenous virus and ALV-E is endogenous virus. ALV-J, a virulent subgroup, is originated from the genetic reassortment between exogenous and endogenous viruses. The prevalence of serum antibody against REV investigated form 2003 to 2005 in breeding chicken in Taiwan was 92.8%. The lesions caused by these three avian lymphoproliferative neoplasm viruses were similar. The lack of classical pathological diagnostic features make it necessary for further differentiation diagnosis. This project is the first attempt to detect and differentiate highly pathogenic and low pathogenicity avian viruses, and the three avian oncogenic viruses infected by MDV, REV, and the ALV subgroups A, J and E. The features of high specificity, high sensitivity, simultaneity, and multiplicity make the microarray become a potential tool for rapid screening and precise diagnosis of these diseases. The implementation of this project could help the control of these important avian diseases and raise the national competitiveness of Taiwan in the poultry industry.高病原性家禽流行性感冒病毒低病原性家禽流行性感冒病毒馬立克病毒家禽白血病病毒網狀內皮細胞增生病毒寡核酸微陣列highly pathogenic avian influenza viruseslow-pathogenicity avian influenza virusesMarek`s disease virusavian leukosis virusavian reticuloendotheliosis virusoligonucleotide microarrays