2009-08-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/711354摘要：百合GRP基因(glycine-rich protein)與MT基因(metallothionein)為灰黴病菌Botrytis elliptica誘發百合系統性抗病以及根圈細菌C1L菌株引發百合系統性抗病的過程中，具有差異性表現之防禦相關基因。基於百合GRP基因在病原真菌及根圈細菌誘導的植株葉片有不同的表現趨勢及先前對抗菌胜&#32957;之觀察，推測百合GRP基因在植物抗性上所扮演的角色與其表現抗菌活性之胜&#32957;有關。MT基因被認為與植物之金屬代謝調控相關，其表現亦可受多種生物與非生物性因子所誘導，尚未有植物病害抗性相關之報導。百合MT基因在病原及根圈細菌誘導之植株葉片均為增加表現，推測百合MT基因的表現可能與植物抗性提升有關。本研究擬利用轉基因植物之建構及農桿菌注入法，分析GRP與MT基因表現對植物抗病能力之影響，驗證GRP與MT基因在植物抗病反應中之功能，並行蛋白質表現及生體外抗菌分析以說明GRP 之抗病功能，並進行基因表現及蛋白質表現之時程及空間分布研究，以增進瞭解此等植物防禦相關基因在誘導抗病反應之參與角色及其於植物保護用途之發展性。<br> Abstract: Defense-related genes of lily GRP (glycine-rich protein) and MT (metallothionein) are differentially expressed in systemic acquire resistance (SAR) elicited by Botrytis elliptica and induced systemic resistance (ISR) caused by rhizobacterium Bacillus cereus C1L. Based on the different expression patterns of lily GRP gene in SAR and ISR, and the previous observation of antimicrobial peptide, we propose that lily GRP gene play a role in disease resistance by expression of antimicrobial trait. As known, expression of MT gene is involved in metal metabolism of plants, and induced by biotic and abiotic factors. Few report on the role of MT gene in disease resistance. However, the increased expression of lily MT gene in both SAR and ISR implicate that this gene have effects on resistance modulation. In this project, we plan to analyze the effects of expressions of GRP and MT genes on plant responses to pathogen attack by construction of transgenic plants and using agroinfiltration method to show the function of GRP and MT genes in disease resistance. In addition, protein expression and antimicrobial assay will be performed to show the function of GRP against pathogen attack. Afterwards, the temporal and spatial patterns of GRP and MT gene expressions and the protein products will be studied. Following these researches, the roles of these plant defense-related genes in induced disease resistance will be better understood and the feasibility of their application in plant protection will be thoroughly evaluated.GRPMT灰黴病菌百合誘導抗病性Glycine-rich proteinmetallothioneinBotrytis ellipticasystemic acquired resistance of lily