2004-01-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/662021摘要：水稻稻熱病（Rice blast）為水稻重要病害。稻熱病乃由Magnaporthe grisea (Hebert) Barr所引起。田間稻熱病菌遺傳變異性極大，具有許多生理小種，也因此水稻抗病品系往往無法維持持久之抗性。為對病害發生之預測、水稻育種篩選及田間施用，提供參考指標，了解田間稻熱病菌之族群及其動態變化，有其必要性。本研究之目標乃針對本土稻熱病菌進行品系分析，基本方向乃利用存在於稻熱病菌中的一重複性序列，稱MGR（Magnaporthe grisea repeat），進行種內分子分型，藉以調查本土稻熱病菌品系之分布及特性，以作為病害發生預測、水稻育種篩選及田間施用之重要參考指標。 本計畫主要目的在於檢定水稻對白背飛蝨危害之感受性，作為田間管理之參考。檢定方法係採用盆栽秧苗人工接蟲以及網室栽培人工接蟲危害，按其危害情況，區分5等級記錄。本項檢定資料可提供因應大量入侵為害，造成衝擊之因應對策，以穩定稻穀生產。<br> Abstract: Rice blast disease is one of the most serious diseases of cultivated rice in many developing countries. When the environmental and climate conditions suitable, the dramatic yield loss will result from the epidemics of the disease. One of the measures to control the disease is deploying cultivars with resistance, however, the breakdowns of the host resistance have often been observed due to the genetic variability of the fungus. To understand the genetic variability of the fungus in Taiwan, we propose to use MGR (Magnaporthe grisea repeat-DNA fingerprinting technique to survey the clone lineages of the fungus. The goal of the proposal is hoping to generate the genetic structure of the fungus in Taiwan and use it to monitor the dynamic structure of the fungus and also as the information for disease prediction, and screening and deploying of resistant rice cultivars. The subject is aimed to test the susceptibility of the major cultivated rice varieties to the white-backed planthopper. Both artificial inoculation of insect pest on seedlings and adult plants of rice according IRRI testing methods (1980) will be adopted in these tests. It is expected to control the pest through the cultivation of resistant variety which is not only can reduce the cost of rice production but also favorable to environment protection.水稻稻熱病菌病原品系調查白背飛蝨抵抗性riceMagnaporthe griseaMGR DNA fingerprintingWhite-backed planthopperResistance