Chen, Hung YuHung YuChenRICHIE CHENPO-CHUAN HSIEHTZONG-JIH CHENG2023-09-192023-09-192023-01-0109106340https://www.scopus.com/inward/record.uri?eid=2-s2.0-85169154291&doi=10.1007%2fs44211-023-00415-4&partnerID=40&md5=e2fd085a89644fbd7dc0d693e8401b88https://scholars.lib.ntu.edu.tw/handle/123456789/635445A new automated, generic analytical approach for determining the clinical disinfectant o-phthalaldehyde (OPA) is reported in this study. The proposed sequential injection analysis (SIA) is based on the online reaction of the OPA with glycine/N-acetylcysteine (NAC) in a neutral medium (pH = 7.0) to form a highly fluorescent isoindole derivative. All critical flow and reaction variables were investigated, while validation was carried out in the linearity detection range (0.0075–0.02%). As a result, excellent linearity (R 2 > 0.99) and precision (1.5–2.4% for repeatability and 0.7–2.2% for reproducibility) were achieved for the reference OPA solutions. Furthermore, reasonable concentration verification of OPA disinfection (0.2–0.6%) in healthcare institutes can be achieved using the developed fluorescent SIA due to its good sensitivity (0.111 V/%) and precision (1.0–2.3% for intermediate precision) around the minimum effective concentration (MEC) of 0.3% for Cidex-OPA disinfectant. Graphical abstract: [Figure not available: see fulltext.]enDisinfectant | Fluorimetry | o-Phthalaldehyde (OPA) | Sequential injection analysis (SIA)[SDGs]SDG3journal article10.1007/s44211-023-00415-4376326462-s2.0-85169154291https://api.elsevier.com/content/abstract/scopus_id/85169154291