2010-08-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/661537摘要：Style 2.1 是控制野生番茄與栽培番茄間雌蕊長度變異的主要因子，此變異亦導致番茄在馴化過程中的自花授粉之生殖系統 (Chen & Tanksley, 2007, Science 318: 643-645)。在野生番茄中，雌蕊的柱頭通常突出於雄蕊管之外或是與雄蕊管等高。而此一形態上的特徵造成一些失去自交不合和基因的野生番茄仍然保有常異交的生殖系統。 由演化發育學的觀點而言，若能確認控制所有番茄雌蕊發育基因的調控網路，將能夠對於由此類生殖系統演化上的限制，有更深入的了解。此一研究將能對於生殖系統的演化、特別是自交是否為生殖系統演化上的死巷，在分子演化方面能有更進一步的認識。此外，就實用層面的考量，位於此調控網路上的基因都將是操作F1雜交種子生產上的最佳目標。 此研究計畫的目的在於發掘能與 style2.1 基因啟動子區域結合的蛋白質。我們將使用 yeast one-hybrid 的技術找出這些蛋白質。未來，期望由找出的蛋白質，建構調控番茄雌蕊長度發育的基因調控網路。 <br> Abstract: Style2.1 is the key factor to control variation of tomato style length between wild tomatoes and cultivated tomato, which lead to self-pollination during tomato domestication (Chen & Tanksley, 2007, Science 318: 643-645). In wild tomato species, stigma is either at the mouth of the stamen cone or protruding outside the stamen cone. This morphological feature results in facultative cross-pollination in most of wild tomato species which lost genetic factor of self-incompatibility. From the view of evolutionary developmental biology, it is important to uncover the entire regulatory pathway controlling style development in tomato, in order to investigate evolutionary constrains for the loss of this reproductive barrier. It would shed light on the evolution of self-pollination as well as on the long-term debates whether self-pollination is evolutionary dead end. For practical consideration, uncovering the style2.1 regulatory pathway also provides new targeting genes to manipulate tomato mating system, which can be useful for the production of F1 hybrid seeds in tomato. This proposal aims to indentify proteins which bind to the style2.1 promoter region. Yeast one hybrid system will be used to retrieve proteins binding to the style2.1 promoter region. We expect to identify proteins that regulate the style2.1 gene, and then construct the gene regulatory pathway for the development of tomato style.番茄雌蕊長啟動子調控因子演化tomatostyle lengthpromoterregulatory factorsevolution