骨科SHAN-HUI HSUChu, W.-P.W.-P.ChuLin, Y.-S.Y.-S.LinChiang, Y.-L.Y.-L.ChiangChen, D.C.D.C.ChenTsai, C.-L.C.-L.Tsai2018-09-102018-09-102004http://www.scopus.com/inward/record.url?eid=2-s2.0-3042519184&partnerID=MN8TOARShttp://scholars.lib.ntu.edu.tw/handle/123456789/307082The effect of a recombinant RGD (arginine-glycine-aspartic acid)- containing fusion protein, cellulose-binding domain ( CBD)RGD, on the cellular adhesion to a biomedical polyurethane (PU) was evaluated. A series of different cell lines, as well as freshly harvested animal cells, were grown on the PU surfaces with or without CBD-RGD, in serum or serum- free media. The results showed that the enhancement of cellular attachment by CBD-RGD varied with cell types. This is believed to be a result of the unique integrin receptors on each type of cell surface. The existence of certain divalent ions (Mg2+ and Mn2+) may increase the efficacy of the CBD -RGD, in a cell type-dependent manner. The fusion protein was also found to inhibit the platelet activation. The effect of CBD-RGD was further examined in two other substrate materials, poly(L-lactide) (PLLA) and poly (lactide -co-glycolide) (PLGA). The effect on cellular adhesion correlated with the amount of CBD-RGD physically adsorbed on the material surface. ( C) 2004 Elsevier B.V. All rights reserved.RGD-containing peptidespolyurethanepoly(L-lactide)poly( lactide-co-glycolide)journal article10.1016/j.jbiotec.2004.03.014