2015-05-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/657073摘要：本設施計畫的主要目標為「創建一個國家級的功能性基因體學研究核心,提供使用者所需的體學服務,藉以厚植台灣生醫實力,提升台灣生技產業競爭力」。我們將提供最先進且完整的功能性基因體學研究平台、一站式單一窗口服務、客製化服務和整合性生物資訊與生物統計服務，並以產學合作為計劃發展重心之一，培育及訓練生技產業所需要的人才。在這個計畫中,除了目前核心實驗室已提供的服務外。今年,在新一代定序系統上,將正式提供最新中通量定序系統 (NextSeq) 、第三代定序系統(Pacific Biosciences)、第四代定序系統 (Ion Proton)服務與數位化PCR(QX200 and RainDrop)。我們將持續建立最新的新一代定序系統技術服務核心設施使用者。今年,核心將擁有最先進的新一代定序系統(一套Illumina NextSeq、一套Illumina MiSeq、一套 Ion Proton、一套 Pacific Biosciences 和一套RainDance Thunder Storm)可以提供使用者台灣最完整與多樣性的新一代定序服務。Ion Proton 有最快的運轉速度,每次上機時間小於 3 小時。Pacific Biosciences 可讀取世界上最長的序列,達 10,000 鹼基,同時可以作單DNA 分子定序。RainDance 是最新的 NGS 技術,對於特定序列 DNA 定序實驗,RainDance 提供了目前為止最完整的 DNA 擷取技術,此三項基因體學服務,截至目前,只有本核心提供服務。數字PCR 系統可用於偵測標的核酸分子,建立精確度和靈敏度的絕對指標。本核心所提供的兩套數位化 PCR 系統分別可產生兩萬顆與千萬顆分割油滴,單一油滴為一個獨立PCR 反應,可分別用於計數與分析。本核心致力於數位化 PCR 系統建置,已可用於多項分子檢測,包括單一反應進行單一與多重基因偵測(singleplex and multiplex)、單核苷酸多態性圖譜、片段化核酸基因拷貝數、低頻腫瘤基因檢測與表達圖譜等,可提供使用者更完整基因體學服務於基礎與轉譯醫學研究。另一方面，於第一期生技類核心設施平台維運計畫的後期，本核心即加強產學合作佈局，計有五個業界合作案進行中‧．新一期計劃中將更積極建立產學合作，並朝協助台灣生技產業升級而努力‧．本核心持續培訓專案管理人員與ISO 17025 認證的相關人員，並協助實驗室同仁取得專案管理證照以符合生技業界的人才需求‧．在過去的三年間，本核心的服務如下，100-101 年度服務收入有新台幣97,942,413 元，102 年度結算有新台幣33,926,855。核心與使用者發表了論文篇數100-101 年度有55 篇、102 年度迄今有28 篇。C5 核心於100-101 年度也申請了7 個專利、102 年度迄今有1 個專利、吸引了一個6,000,000廠商出資的產學計畫，同時培育了超過100 位碩博士生。我們相信本核心將可以滿足國內研究人員的迫切服務需求，協助這些研究同仁達成他們最好的基因體研究表現。<br> Abstract: The main purpose of this project is to establish a national-level functional genomics center which cansupport the state-of the-art services for researchers to strengthen the basis of medical sciences and thecompetitive ability of Taiwan biotech industry. We provide advanced and integrated functional genomictools, one-stop service, customized service and integrated bioinformatics and biostatistics analysis. We alsofocus on academia-industrial cooperation and education for meeting industrial requirements. Except theNCFPB C5 Core Facility provided service items we provide the third generation sequencing, the forthgeneration sequencing and digital PCR services. This year, we continuously establish the up-to-date NGSassay systems to service users. C5 Core Facility possessed several of NGS platforms including one NextSeq,one MiSeq, one Ion Proton, one Pacific BioScience and one RainDance Thunder Storm to service userscompletely and deliver NGS service. IonProton possesses high turnover rate and each assay could completewith 3 hours. Pacific BioScience can read single sequence up to 10,000 bases and can be applied to singleDNA molecule sequencing. RainDance is the latest NGS technology. For specific regions or genessequencing, RainDance offers the latest capture technology. Up to now, these three genomics services areonly provided in C5 Core Facility. Digital PCR system provides an absolute measure of target nucleic acidmolecules with unrivaled precision and sensitivity. C5 Core Facility provides two digital PCR systemswhich can generate twenty thousand and million nanoliter-sized droplets to partition the samples. Eachdroplet is an independent PCR reactor and could use to count positive and negative reaction. C5 CoreFacility focused on digital PCR System establishment and can applied to several applications includingsingleplex and multiplex detection, SNP detection, copy numbers variation in fragmented DNAs, raremutation detection which provide users more complete genomics service for basic and translationalresearches. C5 core facility will pay more attention on academia-industrial cooperation and training ofhigh-level biotech manpower for industrial need. We are having 5 cooperation cases in progress. Our staffsare trained for ISO17025 and practice for project management to get Project Management Professional(PMP) certification. In the past three years, the service income of 2011-2012 is NT 97,942,413 and theservice income of this year is NT 33,926,855. Core members and users of this core are published 83 articles(2011-2012:55 articles; 2013:28 articles) and applied 8 internal patterns (2011-2012:7 patterns; 2013:1patterns). One NT 6,000,000 academic-industry collaboration and formation of two research teams isinitiated by C5. We also cultivate more than 100 Master/PhD students. We believe that this core will satisfythe urgent needs from NSC/NRPB researchers and assist the researchers to make their best achievement.基因體新一代定序數位化PCR一站式單一窗口服務客製化服務GenomicsNGSDigital PCROne-stop servicecustomized service