國立臺灣大學電子工程學研究所管傑雄2006-07-262018-07-102006-07-262018-07-102005-07-31http://ntur.lib.ntu.edu.tw//handle/246246/20036本研究架構了正立式與倒立式的兩種內嵌光鉗差動共焦顯微鏡，詳細描述系 統的架構與元件規格與光路設計。在正立式光鉗架構中，量得了光鉗的Q 值， 並進行了位移偵測的實驗，計算出光鉗的彈力常數k。在倒立式光鉗差動共焦顯 微鏡的架構中，使用了四象限二極體進行橫向位移偵測，結合差動共焦顯微鏡的 縱向解析率，得到了小球三度空間的定位能力。校正結果顯示X、Y、Z 三軸的 解析率分別為為8 nm 、10 nm 與5 nm，同時各具有1 μm 的動態範圍。而量測 小球在光鉗中同時受布朗運動擾動的頻譜訊號，由勞倫茲分佈的擬合曲線截止頻 率可推估水的黏滯係數，並與一般標準值誤差約在1%左右，驗證了本系統可以 小球的動態頻譜分析，得到相當精準的黏滯度估算。Upright and inverted configurations of optical tweezer embedded differential confocal microscopes are constructed. The Q value and the spring constant k of optical tweezer are characterized in the upright configuration system. In the setup constructed with inverted microscope, a quadrant photodiode is used to perform detection of lateral displacement. Combining with the depth resolution power of differential confocal microscope, three-dimensional positioning of a microsphere has been verified. Within 1-μm dynamic range in three axes, the resolutions of X, Y, and Z axes are 8 nm, 10 nm, 5 nm, respectively. By measuring the power spectrum density of silica bead trapped in optical tweezer, the cut-off frequency of Lorentz distribution is fitted. With the cut-off frequency and the spring constant k, the coefficient of viscosity of water is estimated in the experiment, of the error within 1% from the standard value.application/pdf571253 bytesapplication/pdfzh-TW國立臺灣大學電子工程學研究所內嵌光鉗差動共焦顯微鏡黏滯係數optical tweezer embedded differential confocal microscopes are constructedcoefficient of viscosityreporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/20036/1/932213E002074.pdf