Lai, Chen-YenChen-YenLaiLin, Shin-YingShin-YingLinWu, Chia-KaiChia-KaiWuYeh, Li-TzuLi-TzuYehSytwu, Huey-KangHuey-KangSytwuSHI-CHUEN MIAW2019-08-062019-08-062012-08-150022-1767https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864823874&doi=10.4049%2fjimmunol.1200405&partnerID=40&md5=ae10c29c79aff2f84548d7e166f6eee9https://scholars.lib.ntu.edu.tw/handle/123456789/416271Maf proteins are involved in a variety of biological processes, such as oncogenesis, lens development, and differentiation. In immune system, c-Maf transactivates IL-4 promoter, and ectopic expression of c-Maf skews primary T cell response toward the Th2 pathway. Numerous transcription factors are subjected to posttranslational modification. In this study, to our knowledge, we show for the first time that c-Maf is subjective to tyrosine phosphorylation in Th cells and that the level of its tyrosine phosphorylation positively correlates with IL-4 expression by peripheral Th cells, but is negatively associated with the severity of disease in NOD mice. c-Maf undergoes tyrosine phosphorylation at Tyr(21), Tyr(92), and Tyr(131) residues in Th2 cells. Furthermore, tyrosine phosphorylation at these three residues is critical for the recruitment of c-Maf to IL-4 promoter and IL-4 production in Th cells. Taken together, this study sheds new light on the role of posttranslational modification of c-Maf in IL-4 production and Th cell-mediated autoimmune diseases.enjournal article10.4049/jimmunol.1200405227986722-s2.0-84864823874WOS:000307216000004https://api.elsevier.com/content/abstract/scopus_id/84864823874