Wang, Yu-ChiYu-ChiWangFu, Ru-HueiRu-HueiFuHsieh, Hsyue-JenHsyue-JenHsiehChao, Hsiang-TaiHsiang-TaiChaoKao, Shu-HueiShu-HueiKao2008-11-262018-06-282008-11-262018-06-282005http://ntur.lib.ntu.edu.tw//handle/246246/87248Objective: To induce apoptosis of endometriotic cells of patients with endometriosis. Design: To demonstrate that polyglycolic acid/chitosan glue directly inhibits cell proliferation by inducing apoptosis. Setting: University hospital infertility center. Patient(s): Twelve women who visited the center for infertility therapy. Intervention(s): Polyglycolic acid/chitosan glue was applied into primary endometriotic cells; the manipulated cells were collected 1-4 days after polyglycolic acid/chitosan glue treatment. Main Outcome Measure(s): Primary endometriotic cell cultures from eutopic endometriotic tissue were established. The effect of the novel biological glue, polyglycolic acid/chitosan glue A, on endometrial cells in vitro was examined. The different stages of apoptosis were analyzed using flow cytometry with fluorescein isothiocyanate conjugate (FITC)-annexin V and propidium iodide staining. Result(s): The growth inhibitory effects of polyglycolic acid/chitosan glue A on endometrial cells were found to be dose-response and time dependent. Less than 15% viability was detected in cultures containing 2,000 μg of polyglycolic acid/chitosan glue A after 4 days of treatment. Induced apoptosis and caspase activity were revealed. The caspase-3 activity increased 2.2-fold with 4 days of culture with 2,000 μg of polyglycolic acid/chitosan glue A. Conclusion(s): This is the first study to demonstrate that polyglycolic acid/chitosan glue directly inhibits cell proliferation by inducing apoptosis, thus suggesting that this new biological glue may be useful for endometriosis therapy. ?2005 by American Society for Reproductive Medicine.application/pdf415306 bytesapplication/pdfen-USApoptosis; Chitosan; Endometriosis; Polyglycolic acid[SDGs]SDG3caspase 3; chitosan; fluorescein isothiocyanate; glue; lipocortin 5; polyglycolic acid; propidium iodide; apoptosis; article; cell culture; cell proliferation; cell viability; clinical examination; conjugate; controlled study; dose response; endometriosis; endometrium cell; enzyme activity; female; flow cytometry; human; human cell; human tissue; infertility therapy; priority journal; staining; time; university hospital; Apoptosis; Cell Proliferation; Cells, Cultured; Chitosan; Dose-Response Relationship, Drug; Endometriosis; Endometrium; Female; Humans; Polyglycolic Acidjournal article10.1016/j.fertnstert.2005.01.1272-s2.0-22244433561http://ntur.lib.ntu.edu.tw/bitstream/246246/87248/1/09.pdf