2011-01-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/662976摘要：稻熱病為水稻最重要病害之一，於全球各產區均造成相當危害。目前水稻或其他作物病害的監測預警工作，主要係由植物保護或農業相關人員，按時赴田間調查，再依據目視法所觀察到的發病程度，判斷是否發布病害防治警報。此等行之有年的傳統監測模式，其最大限制，在於必須待田間作物開始出現病徵，並達到相當程度後，才足以被調查人員發現；而不同栽培品種上抗感病反應的差異，也容易造成經驗不足的調查人員判斷上的困難，以及監測數據之誤差。本計畫將以稻熱病為模式，發展出不同於傳統目視調查法之創新監測技術，主要工作包括研發簡便的真菌孢子蒐集器，及能準確測定微量稻熱病菌孢子之專一性探針及定量方法，並透過新監測技術的小規模實施，探討以分子生物技術輔助稻熱病及其他空媒病害主動監測工作之可行性。<br> Abstract: Rice blast, caused by Magnaporthe grisea, is one of the most devastating diseases of rice worldwide. Nationwide plant disease monitoring and notification has long been relying on periodic surveys and visual evaluations by trained plant protection personnel. The conventional naked-eye examination approach, however, has its limitations. With visual examination, detection of the onset of an epidemic can be delayed, as the disease severity has to exceed a certain threshold so that the diseased tissues can be seen by the investigator. In addition, monitoring data resulted from visual examinations in different areas are not comparable, because disease occurrence and lesion types can be affected by differential resistance performances of variable plant cultivars. Using rice blast as a model system, this research proposal aims to develop new monitoring technique(s) that will facilitate crop disease forecasting and management. The main objectives include designing aneasy-to-use spore trap for convenient sampling of airborne spores in the field, designing primer pairs for specific detection of M. grisea, and developing a real-time PCR (qPCR) - based method for accurate quantification of M. grisea conidia. By conducting spore trapping combined with qPCR in a small-scale field trial, we will be able to assess the applicability and efficiency of the novel technique in monitoring rice blast as well as other airborne diseases.稻熱病空中捕孢即時定量聚合&#37238連鎖反應Magnaporthe griseaspore trapquantitative real-time PCR