葉力森2006-07-262018-07-092006-07-262018-07-091999http://ntur.lib.ntu.edu.tw//handle/246246/28674Limb allotransplantation is a combination of composite tissues including a great number of highly antigenic and immunocompetent tissues. In animal model study, limb grafts usually encounter uncontrollable rejection and other complications including graft-versus-host disease (GVHD). In our recent studies the rejection of limb grafts may be successfully controlled by combination of novel immuno suppression agents with less side- effects. In this study, limb composite tissue allotransplantation is carried out with a combination of immunosuppressants , bone marrow transplantation, and chemo T cell depletion agents. As a result, this model did not develop a reliable GVHD model, however, some animals developed tolerance after stopping immunosuppressants when the agents were with enough dosage. Under the condition of underdose immunosuppression, no microchimerism was accomplished, and the bone marrow cells may lead to early rejection even GVHD. A further refined immunosuppression protocol is mandatory in future composite tissue trnasplantation.異體肢體移植屬於複合組織移植的一種，其中包含眾多抗原性極強及具免疫競爭力的組織，因此使得在控制移植復的排斥反應非常困難，而且會有移植復的併發症：移植物一宿主病（graft 一versus 一host disease , GVHD ）。本計畫欲以已建立的大鼠異體肢體複合組織移植模式為基礎，模擬臨床運用的情形，以不同劑量之免疫抑制劑合併骨髓移植及化學治療劑，進行異體移植，觀察移植物存活及GVHD 之發生情形，結果顯示，本模式並無法產生可靠之GvHD ，但在某些個體於免疫抑制劑停藥之復，有免疫耐性之產生，在免疫抑制不足情況下，無法產生微細胞交移，骨髓對graft 而言反而是有害的。達成長期存活的首要條件即為足夠的免疫抑制。免疫抑制不足且rec 中ient 免疫系統正常情況下，低劑量免疫抑制的骨髓移植是容易造成graft failur 。且致敏化rec 中ient 免疫系統，甚或是GVHD 。因此免疫抑制計劃的方式及藥物的選擇在未來有重新評估的必要。application/pdf70910 bytesapplication/pdfzh-TW國立臺灣大學獸醫學系暨研究所Limb allotransplantationGraft- versus-host disease(GVHD)Monoclonal antibody(mAb)T cell depletion異體肢體移植移植物一宿主病單株抗體T 淋巴球廓清法reporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/28674/1/882313B002081.pdf