孔祥智老師臺灣大學：免疫學研究所陳香儒Chen, Hsiang-JuHsiang-JuChen2007-11-292018-07-092007-11-292018-07-092004http://ntur.lib.ntu.edu.tw//handle/246246/63325在尋找誘發IL-4表現之相關分子的研究上，小鼠EL4 T 細胞瘤在本研究中發現是一個很好的研究工具。針對EL4 T 細胞瘤的研究發現當細胞瘤經由TCR活化後細胞瘤本身會有IL-4表現，然而該表現能力會隨體外培養時間增長而降低。但是若將細胞瘤重新注入小鼠腹腔中，之後取出細胞瘤並在Mouse EL4 thymoma cells were used as a model system to study TCR-stimulated IL-4 gene expression. In vivo- but not in vitro-passaged EL4 cells were found to activate IL-4 gene upon TCR stimulation. Compared against in vivo-grown EL4 cells, in vitro-grown EL4 cells expressed similar levels of CD3-Table of Contents Abbreviation vi Abstract vii Abstract (Chinese) ix CHAPTER I Introduction 1 1.1 Background information and related studies………….. 1 1.2 Objective of the study…………………………………. 7 CHAPTER II Material and Methods..................................... 8 2.1 Mice……………………………………………………. 8 2.2 Cell culture………………….………………………….. 9 2.3 Marker analysis by flow cytometry……………………. 11 2.4 Intracellular staining …………………………………… 13 2.5 Western-blotting……………………………………….. 15 2.6 Preparation of accessory cells…...…………………….. 17 2.7 Activation of EL4 cells………………………………… 18 2.8 IL-2/IL-4 bioassay…………………………………….. 19 2.9 RNA extraction and RT-PCR………………………….. 21 2.10 Quantitative real-time RT-PCR………………………… 23 CHAPTER III Experimental Results………………………. 25 3.1 Comparison of surface differentiation antigens between in vivo- and in vitro-grown EL4.……………………………….. 25 3.2 Anti-CD3 induced increase in CD25 and CD69 expression in in vivo- EL4 but not in in vitro-grown EL4 cells………… 25 3.3 Similar CD3610838 bytesapplication/pdfen-US小鼠EL4 T 淋巴瘤細胞株EL4otherhttp://ntur.lib.ntu.edu.tw/bitstream/246246/63325/1/ntu-93-R91449002-1.pdf