CHIH-HSIEN CHIULin, Wen-DerWen-DerLinHuang, Shao-YongShao-YongHuangLee, Ying-HueYing-HueLeeLin, W.D.W.D.LinHuang, S.Y.S.Y.Huang2019-09-262019-09-262004-08-150890-9369https://scholars.lib.ntu.edu.tw/handle/123456789/425349CCAAT/enhancer-binding proteins, C/EBPalpha and C/EBPbeta, are required for fat cell differentiation and maturation. Previous studies showed that replacement of C/EBPalpha with C/EBPbeta, generating the beta/beta alleles in the mouse genome, prevents lipid accumulation in white adipose tissue (WAT). In this study, beta/beta mice lived longer and had higher energy expenditure than their control littermates due to increased WAT energy oxidation. The WAT of beta/beta mice was enriched with metabolically active, thermogenic mitochondria known for energy burning. The beta/beta allele exerted its effect through the elevated expression of the G protein alpha stimulatory subunit (Galphas) in WAT. Galphas, when overexpressed in fat-laden 3T3-L1 cells, stimulated mitochondrial biogenesis similar to that seen in the WAT of beta/beta mice, and effectively diminished the stored lipid pool.enC/EBP; gene replacement; G alpha(s); WAT adipocyte; mitochondrial biogenesisjournal article10.1101/gad.1213104152894642-s2.0-4043164343WOS:000223303600007https://api.elsevier.com/content/abstract/scopus_id/4043164343