Chen, Pin ChunPin ChunChenCHIH-TIEN WANG2022-12-142022-12-142023-01-01978-1-0716-2670-2978-1-0716-2671-910643745https://scholars.lib.ntu.edu.tw/handle/123456789/626424PC12 cells serve as a secretory cell model, especially suitable for studying the molecular mechanisms underlying fusion pore kinetics in regulated exocytosis of dense-core vesicles (DCVs). In this chapter, we describe a series of PC12 cell culture procedures optimized for real-time functional assays such as single-vesicle amperometry. In addition, these conditions have been widely used for single-cell biochemical assays such as the proximity ligation assay with immunostaining.enDense-core vesicle | Fusion pore kinetics | Norepinephrine | PC12 cell culture | Proximity ligation assay with immunostaining | Rat pheochromocytoma | Regulated exocytosis | Single-cell biochemistry assay | Single-vesicle amperometrybook part10.1007/978-1-0716-2671-9_1362058832-s2.0-85139570654https://api.elsevier.com/content/abstract/scopus_id/85139570654