HSIAO-MEI WUChang, Wei JenWei JenChangLee, Tse AngTse AngLeeLiao, Wei HaoWei HaoLiaoTung, Yi ChungYi ChungTung2023-07-312023-07-312021-01-019781733419031https://www.scopus.com/inward/record.uri?eid=2-s2.0-85136978873&partnerID=40&md5=53572902f88bd5cdd79cac8dd4017997https://scholars.lib.ntu.edu.tw/handle/123456789/634270Oxygen affects cellular responses in different aspects, e.g., metabolic pathways and plasma membrane integrity. Accurate measurement of intracellular oxygen concentration becomes an essential task in biomedical research. Due to the advantages provided by the widefield technique, the image acquisition speed of the system is faster and the exposure time can be reduced significantly. Therefore, it is suitable to be applied for time-lapsed observation to study transient phenomena. Using a novel microfluidic device, we can control the microenvironmental environment and real-time monitor the intracellular oxygen responses. Through this measurement, the cellular response to rapid oxygen microenvironment variation can be observed.Fluorescence Lifetime Imaging Microscopy (FLIM) | Intracellular Oxygen Response | Microfluidic Devices | Oxygen Microenvironmentconference paper2-s2.0-85136978873https://api.elsevier.com/content/abstract/scopus_id/85136978873