2006-01-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/663272摘要：利用誘導抗病性以防治植物病害之研究工作當中，發現施用水楊酸及樸殺熱可以降低百合感染灰黴病的嚴重度，在選殖水楊酸誘導之互補核酸中，鑑定一解碼富含甘胺酸蛋白質之互補核酸LsGRP1，由相似蛋白質之研究推測LsGRP1之表現與植物抗病性有關，可作為分子抗病育種之基因資源。為確定此基因於增進植物抗病功能之潛力，進行一系列功能性研究，包括利用農桿菌滲入或病毒載體導入方式使導入核酸序列表現於植物組織，由LsGRP1之表現對灰黴病菌感染之影響來得知所測試富含甘胺酸蛋白質與植物抗灰黴病的關係。<br> Abstract: Salicylic acid and probenazole has been found to decrease disease severity caused by Botrytis infection on lily. LsGRP1 cDNA has been cloned from Lilium 'Star Gazer', which are related to disease resistance based on amino acid sequence analysis and comparison and can be the gene resource for molecular breeding of disease resistance.To verify the relatedness of LsGRP1 and disease resistance of lily against Botrytis blight, the LsGRP1 funtion will be analyzed. The LsGRP1 wil be constructed in the Agrobacterium vector or viral vector to express in 'Star Gazer' leaves. The transient expression assay will be performed to examine the expression of LsGRP1 construct in 'Star Gazer' leaves and its effect on the Botrytis infection.葵百合富含甘胺酸蛋白質植物抗病性農桿菌注入法LsGRP1plant disease resistanceagroinfiltration