2007-01-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/678304摘要：我們對蚯蚓這種生活在我們周遭的土壤動物，尤其是台灣蚯蚓的瞭解實在太少了。一百多年前，演化學之父達爾文即曾對蚯蚓爬出地面被曬死的現象，感到困惑；紫外線可能是主因的想法在30年代被提出，而在我們的先前研究中也確定了紫外線是造成蚯蚓死亡的主因之一。 目前有許多紫外線對動物細胞的研究，但對於個體的研究卻相當缺乏，由過去的研究報告中也發現，紫外線對生物體會產生許多傷害，包括DNA damage，細胞膜的損害，致癌機率的增加等，其中也發現紫外線會造成細胞的程式死亡，其中一個引發機制是細胞氧化壓力的增加，嚴重時會造成生物的損害或死亡，因此本實驗計畫以台灣常見的兩種蚯蚓為對象，分別是體色較深並對紫外線敏感的Amynthas gracilis，及體色較淺並對紫外線較不敏感的Pontoscolex corethrurus，來探討紫外線是否會經由氧化壓力這條途徑引發細胞程式死亡並傷害蚯蚓，同時NO及NOS是否與造成氧化壓力及細胞凋亡有關，同時比較這兩種對紫外線反應不同的物種中，其氧化機制是否有差異，並建立以蚯蚓作為紫外線研究的模式生物。 <br> Abstract: The knowledge about the neighboring soil animal, earthworm was little known especially the earthworms in Taiwan. In 1888, Darwin had ever confused about why the earthworms come out and die by drying on the soil surface; Scientists proposed that UV can anesthetize earthworms causing their death. According our previous study, we have demonstrated that UV is a key factor to kill earthworms. There are many reports about the UV damages to animal cells (in vitro), but the researches to animal systems (in vivo) are few. Its hazardous health effects include DNA damage, cell membrane harmful and the generation of skin cancer. One of these damages is program cell death. With respect to UV-induced apoptosis, the direct mechanism is increasing reactive oxygen species (ROS) that causes animal damage or dead. In order to understand whether UV induced program cell death in earthworms caused by ROS, two common species of earthworm in Taiwan, Amynthas gracilis (dark color and sensitive to UV) and Pontoscolex corethrurus (light color and insensitive to UV), will be used as study animals. Besides, NO (nitric oxide) and NOS (NO synthanase) were studied to understand the relationship between apoptosis, oxidative stress and NO. We will compare the damages in these two species to research the mechanism of ROS in animals. Hopefully, the earthworm could be the model animal for UV study in the future.紫外線蚯蚓一氧化氮細胞程式死亡ultravioletearthwormnitric oxideapoptosis