Wang C.-H.Lin J.-H.Lu T.-J.Chiang A.-N.Chiou S.-T.Chen Y.-A.Pan M.-H.SHU-CHEN HSIEHMIN-HSIUNG PAN2019-07-162019-07-16201300218561https://scholars.lib.ntu.edu.tw/handle/123456789/414032Cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) are transcriptional targets of nuclear factor kappa B (NF-£eB) that are involved in inflammatory responses. The aim of this study is to develop a method for efficiently detecting inflammation modulatory activities. Here we established RAW264.7 macrophage cells stably expressing a luciferase reporter gene directed by iNOS or COX-2 promoter. Lipopolysaccharide (LPS) treatment stimulated the luciferase activity which paralleled with increased iNOS and COX-2 mRNA levels determined by RT-q-PCR. The LPS-stimulated luciferase activity was blocked by NF-£eB inhibitor CAPE and by nobiletin, an anti-inflammatory natural product from citrus peels. We have applied the platforms to screen various mushroom species; analysis by scatter plot revealed a strong correlation to the results obtained by ELISA-based detection of TNF-£\. Together we have established luciferase reporter systems sensitive to NF-£eB-dependent iNOS and COX-2 activation, which provides an alternative screening method for identifying food components with immune-modulatory activities. ? 2013 American Chemical Society.cell platformCOX-2iNOSluciferase assayRAW264.7journal article10.1021/jf404887u2-s2.0-84891390463https://www.scopus.com/inward/record.uri?eid=2-s2.0-84891390463&doi=10.1021%2fjf404887u&partnerID=40&md5=e5087ea9129bad2c8bc802c237ed45c6