2023-01-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/666254即時聚合酶連鎖反應(real time polymerase chain reaction, rtPCR)是目前廣泛用於檢測新型冠狀病毒(SARS-CoV-2)的方法，雖然具有高靈敏性，但卻也有著樣品需求大且機器昂貴不便攜帶等缺點。不過，微加工技術的發展，則使多團隊得以製作微型PCR裝置。微流道的高表面積與體積比，除可大幅減少樣品量，也可加速溶液的溫度循環，從而實現低耗能卻快速的PCR。然而，目前微型PCR的研究仍有許多問題存在。PCR熱源供應、流道內溫度均勻分布，及同時多種DNA片段放大與即時檢測等，均是要克服的問題。此前，本團隊已於國際期刊發表一整合SPR晶片與微流道PCR的裝置，並驗證該裝置可檢測真實樣品之基因。不過該裝置是以金屬加熱板為PCR熱源；此熱源限制裝置的微小化。 The polymerase chain reaction (PCR) is widely used to detect SARS-CoV-2. Although PCR is highly sensitive, it has the disadvantage of requiring a large sample size and costly equipment. However, the development of micro electro-mechanical systems (MEMS) has made it possible for researchers to construct tiny devices. The microchannel`s high aspect ratio of surface to volume minimizes sample requirements and boosts the heating cycle rate, resulting in more efficient energy utilization. Nonetheless, microfluidic PCR still faces obstacles, such as the PCR heating source, the temperature distribution in the microchannel, and the simultaneous detection of numerous DNA sequences. Our team has previously exhibited systems using SPR sensors and microfluidic PCR that can identify DNA extracted from actual samples. However, this gadget is heated by a metal plate, limiting the device`s potential for volume reduction. To address the issue, this study focuses on developing a novel device using microfluidic photonic PCR and image surface plasmon resonance (image SPR). Introducing photonic PCR can reduce the size and energy consumption of the device. In addition, the SPR system can image PCR data, allowing for a greater detection limit, a lower signal-to-noise ratio, and a high throughput analysis. This device can perform rapid PCR thermocycling with light and concurrently detect numerous DNA fragments.光子聚合酶連鎖反應;影像化表面共振電漿;新型冠狀病毒;微流道;Photonic PCR; Image SPR; SARS-CoV-2; microchannel