2008-01-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/659255摘要:收集本省之養殖九孔病例,針對&#30129;疹樣病毒,進行分子生物學研究包抬聚合酵素鏈反應和基因建立及篩選適合九孔&#30129;疹病毒之引子對,並建立篩選九孔&#30129;疹病毒pol基因之引子對,並據此應用在臨床及檢疫用。<br> Abstract: Mass mortalities of abalone occurred among cultured abalone in northeastern Taiwan has been reported. Both adult and juvenile abalone suffered from the disease with cumulative mortalities of 70%-80%. Histological examination of moribund abalone indicated that the nerve system was the primary target tissue. Transmission electron microscopy examination of cerebral ganglions demonstrated viral particles within the degenerated cells. The viral particles were hexagonal, 90-100 nm in diameter. The morphology and size of the viral particles were most consistent with a herpes-type virus described from the oyster, Crassostrea virginica. Outbreaks of the disease were noted in the following two years in different farms in the areas and induced high mortality. In the study, the virus will be study using PCR technique associated with pol gene for quarantine purpose.九孔疾病檢疫abalonediseasesquarantine建立動物及其產品輸出入風險評估與傳染病監控體系-九孔&#30129;疹病毒pol基因聚合酵素鏈鎖反應之開發