Sheen L.-Y.Sheu S.-F.Tsai S.-J.Meng R.H.C.Lii C.-K.2019-07-112019-07-1119990192415Xhttps://scholars.lib.ntu.edu.tw/handle/123456789/413595This study investigated the effects of various concentrations and incubation time intervals of diallyl disulfide (DADS), the active principle of garlic, on: 1. cell viability, 2. lipid peroxidation. and 3. glutathione (GSH) concentration and its related enzyme activities of rat hepatocytes. According to the results of LDH leakage and microscopic examination, 0.5 and 1 mM DADS did not significantly affect the viability of hepatocytes. However, significant decrease in cell viability according to increased LDH leakage and significant changes in morphology of hepatocytes were observed at 2 mM DADS (P<0.05). Lipid peroxidation was also detected when the hepatocytes were treated with 2 mM DADS. At 0.5 mM DADS, a higher GSH content was found in the hepatocytes although not at a statistically significant level. 0.5 and 1 mM DADS has little effect on the activities of glutathione-Stransferase (GST) and glutathione peroxidase (GPx); however a significant decrease in GST, GPx and glutathione reductase (GRd) activities was observed at 2 mM DADS. Once the media of 2 mM DADS was replaced with fresh medium at 24 hr treatment, the activities of GST, GRd and GPx were recovered, although they were still lower than the control values. ? 1999 Institute for Advanced Research in Asian Scsience and Medicine.[SDGs]SDG6journal article10.1142/S0192415X990001242-s2.0-0032601866https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032601866&doi=10.1142%2fS0192415X99000124&partnerID=40&md5=3f49cc17cad3ffe91b99069d40782c9d