Wang Y.-S.Tsang Y.-W.Chi C.-H.Chang C.-C.Chu R.-M.CHAU-HWA CHI2020-01-072020-01-0720080304-3835https://scholars.lib.ntu.edu.tw/handle/123456789/446375Interleukin-2 (IL-2) and granulocyte-macrophage colony stimulating factor (GM-CSF) facilitate the maturation and functioning of injected DC. We developed a method of in situ electroporation using IL-2 and GM-CSF genes (EGT/cytokines), followed by intra-tumoral (i.t.) immature DC to determine the immune response at the tumor site using prostate-specific antigen-transfected CT26 cells. Three cycles of EGT/cytokines and i.t. DC inhibited tumor growth most effectively, but not superior to EGT/cytokines alone. However, the role of i.t. DC became significant when radiation was given after immunotherapy, which may have clinical implications on achieving better local control and prevention of systemic relapse. ? 2008 Elsevier Ireland Ltd. All rights reserved.[SDGs]SDG3granulocyte macrophage colony stimulating factor; interleukin 2; prostate specific antigen; adjuvant therapy; animal cell; animal tissue; article; cancer combination chemotherapy; cancer gene therapy; cancer immunotherapy; cancer radiotherapy; colon adenocarcinoma; controlled study; dendritic cell; electroporation; female; genetic transfection; immune response; mouse; multiple cycle treatment; nonhuman; priority journal; tumor growth; tumor localization; Animals; Cell Line, Tumor; Combined Modality Therapy; Dendritic Cells; Electroporation; Gene Therapy; Granulocyte-Macrophage Colony-Stimulating Factor; Injections; Interleukin-2; Killer Cells, Natural; Lymphocytes, Tumor-Infiltrating; Mice; Mice, Inbred BALB C; Neoadjuvant Therapy; Neoplasms, Experimental; T-Lymphocytesjournal article10.1016/j.canlet.2008.02.0632-s2.0-45049086553https://www2.scopus.com/inward/record.uri?eid=2-s2.0-45049086553&doi=10.1016%2fj.canlet.2008.02.063&partnerID=40&md5=c167728b9b344974d2050fd3ede45052