Huang S.-HChang Y.-SChang K.-WMONG-HSUN TSAIHuang N.-T.2021-07-262021-07-262020https://www.scopus.com/inward/record.uri?eid=2-s2.0-85079688217&partnerID=40&md5=c597bb744e6fb18fa6bc070f70f848a1https://scholars.lib.ntu.edu.tw/handle/123456789/572954We developed a fully automatic microfluidic platform integrating DNA hybridization processes to reduce the hybridization time and eliminate potential manipulation errors. By using a novel DNA hybridization protocol of adding SYBR green I (SGI) and graphene oxide (GO) sequentially, the signal difference between perfect and non-perfect matches can be enhanced. Our platform can achieve single nucleotide polymorphisms (SNPs) detection in 2.5 hours and has the potential to provide accessible genetic screening for timely clinical treatments. ? 17CBMS-0001.DNA; Dyes; Graphene oxide; Microarrays; Microfluidics; Nucleotides; Clinical treatments; DNA hybridization; DNA micro-array; Genetic screening; Intercalating dye; Microfluidic platforms; Perfect matches; Single nucleotide polymorphisms; Grapheneconference paper2-s2.0-85079688217