Wu, Yi-ChunYi-ChunWuTsai, Miao-ChihMiao-ChihTsaiCheng, Li-ChunLi-ChunChengChou, Chung-JungChung-JungChouWeng, Nei-YinNei-YinWeng2009-06-242018-07-062009-06-242018-07-062001http://ntur.lib.ntu.edu.tw//handle/246246/161408http://ntur.lib.ntu.edu.tw/bitstream/246246/161408/1/02.pdfWe have identified and characterized a novel C. elegans gene, ced-12, that functions in the conserved GTPase signaling pathway mediated by CED-2/CrkII, CED-5/DOCK180, and CED-10/Rac to control cell migration and phagocytosis of apoptotic cells. We provide evidence that ced-12 likely acts upstream of ced-10 during cell migration and phagocytosis and that CED-12 physically interacts with CED-5 and forms a ternary complex with CED-2 in vitro. We propose that the formation and localization of a CED-2-CED-5-CED-12 ternary complex to the plasma membrane activates CED-10, leading to the cytoskeletal reorganization that occurs in the polarized extension of cell surfaces in engulfing cells and migrating cells. We suggest that CED-12 counterparts in higher organisms regulate cytoskeleton dynamics, as CED-12 does in C. elegans. © 2001 Cell Press.application/pdf457226 bytesapplication/pdfen-USjournal article10.1016/S1534-5807(01)00056-92-s2.0-0035487807WOS:000175301500009http://ntur.lib.ntu.edu.tw/bitstream/246246/161408/1/02.pdf