TZU-MING PANHu, Kuang-ChunKuang-ChunHuHsu, LCLCHsuHsu, YWYWHsuWang, Horng-YuanHorng-YuanWangLiang, YHYHLiangLiu, Sung-ChenSung-ChenLiuLiaw, CCCCLiawLiu, Chuan-ChuanChuan-ChuanLiuHung, Chung-LiehChung-LiehHungKuo, YHYHKuoBair, Ming-JongMing-JongBairPan, TM.TM.PanLiu, Chun-JenChun-JenLiuWu, Ming-ShiangMing-ShiangWuShih, Shou-ChuanShou-ChuanShih劉俊人吳明賢2018-09-102018-09-102012http://www.scopus.com/inward/record.url?eid=2-s2.0-84856148338&partnerID=MN8TOARShttp://scholars.lib.ntu.edu.tw/handle/123456789/369440A new azaphilonidal derivative, monapurpyridine A (MPA), has recently been isolated from the fermented products of Monascus purpureus NTU 568. The structure of MPA was elucidated by nuclear magnetic resonance ( 1H-NMR, 13C-NMR, COSY, HMQC, and HMBC) and other spectroscopic analyses. Biological evaluation revealed that MPA could induce cell death in human breast adenocarcinoma cells MCF-7, and it has no significant toxicity to normal mammary epithelial cells M10. The MTT assay and flow cytometric analysis were employed to investigate cell viability and cell cycle influenced by MPA. Moreover, we used Western blot and caspase activity assay to demonstrate the activation of caspase-3, -8 and -9 resulted from MPA. All evidence supported that MPA was suitable for developing into a chemotherapeutic or chemopreventive agent against breast cancer. ? 2012 by the authors.Apoptosis; Azaphilone; Cytotoxicity; Monapurpyridine A; Monascus purpureus NTU 568[SDGs]SDG3antineoplastic agent; caspase; monapurpyridine A; pyridine derivative; adenocarcinoma; apoptosis; article; breast tumor; cell survival; chemical structure; chemistry; culture medium; drug effect; enzyme activation; female; fermentation; human; isolation and purification; metabolism; Monascus; rice; tumor cell line; Adenocarcinoma; Antineoplastic Agents; Apoptosis; Breast Neoplasms; Caspases; Cell Line, Tumor; Cell Survival; Culture Media; Enzyme Activation; Female; Fermentation; Humans; Molecular Structure; Monascus; Oryza sativa; Pyridinesjournal article10.3390/molecules17010664