2005-08-012024-05-16https://scholars.lib.ntu.edu.tw/handle/123456789/669141摘要：臺灣杉 (Taiwania cryptomerioides Hayata) 是臺灣原產重要經濟樹種，與銀杏 (Ginkgo biloba)、世界爺 (Sequoiadendron ginanteum)及水杉 (Metasequoia glyptostroboides) 同屬中生代與新生代第三紀孑遺植物，為世界級之珍貴樹種。就木材利用的角度來看，臺灣杉具有極佳的耐腐朽的樹種，此外，至目前為止已有相當多篇的研究指出臺灣杉之二次代謝產物 (抽出成分) 具有抗真菌、抗白蟻、抗氧化、及抗腫瘤等生物活性。但就分子生物學的角度來看，這些參與臺灣杉生長、發育及二次代謝物的生合成相關酵素研究，則是甚少研究論文。因此本計畫即欲進行臺灣杉功能性基因體之研究，首先我&#20204;將先建構基因庫cDNA library，匯集性狀表現基因（Expressed Sequence Tags，EST；之後，我們將設計及收集不同樹齡、不同品系、不同生育地、或其他不同環境因子 (如溫、濕度、及光線等逆境處理) 的臺灣杉材料，抽取其mRNA，再利用核酸雜交的方式分析所有基因表現的情形，如此將可快速找出哪些基因可能與臺灣杉生<br> Abstract: Taiwania (Taiwania cryptomerioides Hayata) is a native tree that grows at elevations from 1800-2600 m in Taiwan’s central mountains. Taiwania as well as Ginkgo biloba, Sequoiadendron giganteum and Metasequoia glyptostroboides are the living fossils from Tertiary period of Mesozoic Era. It is one of the most economically important tree species grown in Taiwan. To consider in the wood utilization of view, Taiwania is classified as the species with an excellent durability in Taiwan. In addition, there are several papers dealing with the antifungal, antitermite, antioxidiant, and antitumor activities of the extractives isolated from Taiwania. However, investigation of the importance of enzymes in the plant development has been hampered by a lack of information at a molecular level. Therefore, in this proposed project, we will study on the functional genomics of Taiwania cryptomerioides Hayata. Firstly, we will construction the cDNA libraries of Taiwania. Collection the expression sequence tags (ESTs) to be spotted on the DNA ships. Using hybridization to analysis the gene expression at different aged, lines, growth area, and environmental factors (ex temperature, humidity, and light), rapid screening the genes involved in Taiwania development or physiological response. These will obtain the genes associated with differential and metabolism to provide the useful information for the researches on tree physiology, evolution, gene transformation, and drug discovery in the future.臺灣杉功能性基因體性狀表現基因Taiwania cryptomerioides Hayatafunctional genomicsexpression sequence tag (EST)