Huang, C.-Y.C.-Y.HuangTseng, W.-R.W.-R.TsengAhmed, A.F.A.F.AhmedChiang, P.-L.P.-L.ChiangTai, C.-J.C.-J.TaiHwang, T.-L.T.-L.HwangCHANG-FENG DAISheu, J.-H.J.-H.Sheu2018-09-102018-09-102018http://www.scopus.com/inward/record.url?eid=2-s2.0-85044418262&partnerID=MN8TOARShttp://scholars.lib.ntu.edu.tw/handle/123456789/402144Three new polyoxygenated steroids, michosterols A-C (1-3), and four known compounds (4-7) were isolated from the ethyl acetate (EtOAc) extract of the soft coral Lobophytum michaelae, collected off the coast of Taitung. The structures of the new compounds were elucidated on the basis of spectroscopic analyses and comparison of the nuclear magnetic resonance (NMR) data with related steroids. The cytotoxicity of compounds 1-3 against the proliferation of a limited panel of cancer cell lines was assayed. Compound 1 was found to display moderate cytotoxicity against adenocarcinomic human alveolar basal epithelial (A549) cancer cells. It also exhibited potent anti-inflammatory activity by suppressing superoxide anion generation and elastase release in N-formyl-methionyl-leucyl-phenylalanine/cytochalasin B (fMLP/CB)-stimulated human neutrophils. Furthermore, 3 could effectively inhibit elastase release, as well. ? 2018 by the authors.Anti-inflammatory activity; Cytotoxicity; Lobophytum michaelae; Soft coral[SDGs]SDG3[SDGs]SDG1423 norgorgost 5 en 3beta ol; 23 norgorgosterol; brassicasterol; elastase; gorgosterol; idelalisib; methylcholesterol; michosterol A; michosterol B; michosterol C; steroid; superoxide; unclassified drug; antiinflammatory agent; cytochalasin B; cytotoxin; formylmethionylleucylphenylalanine; pancreatic elastase; steroid; superoxide; A-549 cell line; antiinflammatory activity; Article; cell proliferation; controlled study; coral; DLD-1 cell line; drug cytotoxicity; drug efficacy; drug potency; drug structure; enzyme release; human; human cell; IC50; in vitro study; LNCaP cell line; Lobophytum michaelae; neutrophil; Taiwan; animal; Anthozoa; chemistry; drug effect; metabolism; nuclear magnetic resonance spectroscopy; procedures; tumor cell line; A549 Cells; Animals; Anthozoa; Anti-Inflammatory Agents; Cell Line, Tumor; Cell Proliferation; Cytochalasin B; Cytotoxins; Humans; Magnetic Resonance Spectroscopy; N-Formylmethionine Leucyl-Phenylalanine; Neutrophils; Pancreatic Elastase; Steroids; Superoxidesjournal article10.3390/md160300932-s2.0-85044418262WOS:000428510200019