Sun Y.-C.Liou H.-M.PO-TING YEHWEI-LI CHENFUNG-RONG HU2020-10-222020-10-2220170146-0404https://www.scopus.com/inward/record.uri?eid=2-s2.0-85026290845&doi=10.1167%2fiovs.16-20998&partnerID=40&md5=2eb657f486bbdd05dceb06cd35b1462dhttps://scholars.lib.ntu.edu.tw/handle/123456789/517794To determine the presence of monocarboxylate transporter (MCT) in human and rabbit corneal epithelium and its role in transcellular fluorescein transportation in the cornea. METHODS. The presence of MCTs in human and rabbit corneal epithelium was determined by RT-PCR and immunohistochemistry. Intracellular fluorescein uptake experiment was performed using cultured human corneal epithelial cells (HCECs). The involvement of MCT in fluorescein uptake was determined by addition of MCT inhibitors to HCECs and acute dry eye model on New Zealand albino rabbits by spectrophotometry, corneal impression cytology, and external eye photographs. RESULTS. MCT-1 and MCT-4 were identified in both human and rabbit corneal epithelia. A longer treatment period and a lower pH value in culture medium increased fluorescein uptake in HCECs. Fluorescein uptake in HCECs was decreased following addition of MCT inhibitors in a concentration-dependent manner. Impression cytology under fluorescent microscopy showed intracellular fluorescein staining in the rabbit cornea with acute dry eye treatment that was decreased following topical treatment of MCT inhibitors. CONCLUSIONS. Fluorescein ingress in corneal epithelial cells is mediated by the MCT family. Further study of MCT-mediated transport on HCECs may potentially benefit differential diagnosis and contribute better understandings of ocular surface disorders. ? 2017 The Authors.Corneal epithelial cell; Fluorescein staining; Monocarboxylate transporter[SDGs]SDG2[SDGs]SDG3dihydro 4,4' diisothiocyano 2,2' stilbenedisulfonic acid; fluorescein; monocarboxylate transporter; 4,4' diisothiocyanatostilbene 2,2' disulfonic acid; cotransporter; fluorescein; fluorescent dye; monocarboxylate transport protein 1; monocarboxylate transporter; muscle protein; salicylic acid derivative; SLC16A4 protein, human; animal cell; animal tissue; Article; cell culture; controlled study; cornea epithelium; cytology; drug uptake; dry eye; fluorescence microscopy; fluorometry; human; human tissue; immunohistochemistry; nonhuman; pH; priority journal; reverse transcription polymerase chain reaction; RNA isolation; spectrophotometry; transepithelial resistance; acute disease; animal; antagonists and inhibitors; cornea epithelium; dose response; metabolism; pathology; physiology; rabbits and hares; real time polymerase chain reaction; transport at the cellular level; 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; Acute Disease; Animals; Biological Transport; Cells, Cultured; Dose-Response Relationship, Drug; Dry Eye Syndromes; Epithelium, Corneal; Fluorescein; Fluorescent Dyes; Humans; Hydrogen-Ion Concentration; Immunohistochemistry; Microscopy, Fluorescence; Monocarboxylic Acid Transporters; Muscle Proteins; Rabbits; Real-Time Polymerase Chain Reaction; Salicylates; Symportersjournal article10.1167/iovs.16-20998287384152-s2.0-85026290845