2009-01-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/661780摘要：本計畫基於拮抗性臘狀芽孢桿菌菌株可以經由處理根圈得以表現誘導植物系統性抗病的能力，希望研究相關之機制及開發附加之應用價值。擬以拮抗性臘狀芽孢桿菌菌株處理植物根圈及接種病原真菌，篩選拮抗性臘狀芽孢桿菌之植物抗病性誘發因子誘變株，續由轉位子插入位置分析，鑑定與植物抗病誘導能力有關之基因；進行目標基因破壞之變異株的互補試驗，以確定植物抗病性誘發因子之本質，期對拮抗性臘狀芽孢桿菌菌株之誘導抗病能力有更深入的瞭解及延續發展以植物抗病性誘發物為活性組成之植物保護劑。<br> Abstract: This project is based on the systemic resistance-inducing ability of Bacillus cereus. The goal is to unravel the mechanism of systemic induced resistance-driven by B. cereus and to explore the application merit. The transposon-directed mutant library will be constructed and screened on the plants for the loss of ISR-eliciting ability. The genes involved in the ISR-elicitation will be approached by analysis of the transposon-insertion sites. The complementation test on the preliminary ISR mutants will be performed to verify the factors identified to be ISR-related. This study is for better understanding on the ISR-eliciting ability of B. cereus and the development of ISR-eliciting factor as a plant protection agent.臘狀芽孢桿菌轉位子誘變植物抗病性誘發因子互補試驗Bacillus cereustransposon mutagenesiseliciting factors of disease resistancecomplementation test