2006-01-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/684491摘要：非生物性逆境往往限制水稻之生長發育並影響稻米之質量及產量。由於水稻之非生物性逆境耐受性之性狀多為數量遺傳基因所控制，傳統之育種改良進展較慢且有極限。隨箸後基因體世代的來臨，功能性基因體學如T-DNA 插入所產生之水稻突變系成為研究水稻不同基因其生理功能與品質、農藝性狀、產量等之重要工具。本計畫為利用水稻T-DNA插入突變系進行發育與抗逆境功能性基因體研究子計畫之一，目的即在配合其它子計畫進行水稻抗逆境相關突變系農藝性狀及生理、生化等分析。主要項目包括突變株插入DNA序列之鈞取，同型結合突變株之篩選及分子鑑定，以及突變株之生理、生化及農藝性狀分析等。本計晝預期可針對不同具GUS染色之T-DNA突變株釣取插入性DNA序列，並進行基因比對。並可就篩選到與高鹽、乾旱及溫度逆境有關之同型結合突變株，分析其表現之性狀與生理功能。最後可由研究結果提昇吾人對水稻逆境耐受性機制的瞭解，進一步提供水稻育種或栽培之參考。其中確認功能之有用基因亦可進一步申請專利提供商業利用價值。<br> Abstract: Abiotic stress is known as a major factor that limits crop growth and development and affects crop production and quality. Most of abiotic stress tolerance teaits in crops are controlled by qualitative trait loci (QTL). The improvement of crop abiotic stress tolerance by conventional breeding or cultivation is quite slowly and has its own limitation. Recently since the decoding of rice genome, many functional genomic tools are available, especially for T-DNA insertional mutagenesis rice mutant pools. This project is a sub-project of functional genomics study of rice development and abiotic stresses with T-DNA insertional rice mutants. The main goal of this project is to analysis the physiological characteristics of abiotic stress related rice mutants found by other teams. We aimed at studying isolation of flanking sequences of GUS-positive staining T-DNA lines, molecular characterization and PCR-baesd screening of homozygous mutant lines, and determine the agronomical traits, physiological and biochemical responses of these mutants under abiotic stresses. Results obtained from the above research are expected to increase our current understanding of rice abiotic stress tolerance mechanism, and serves as a foundation for crop breeders. Besides, the validation of gene-fuctions from these experiments will be used further for patten application and commercial use.水稻突&#21464體高及低&#28201T-DNA插入功能性分析rice mutantshigh and low temperatureT-DNA insertionfunctional analysis