2005-07-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/682577摘要：象鼻蟲之分類隸屬於鞘翅目多食亞目象鼻蟲總科，其特徵為發達的咀嚼式口器，連同部分的頭部，形成長鼻狀。他們會蛀食根、果實以及植物莖幹，對農作物造成危害。由於許多種類之象鼻蟲將卵產於植物組織內，造成施藥效果不彰與檢疫之困難。目前我國列為檢疫防疫害蟲之象鼻蟲計有 (1) 檬果種子象鼻蟲(Acryptorrhychus mangiferae)；(2) 檬果象鼻蟲 (A. frigidus)；(3) 李象鼻蟲 (Conotrachelus nenuphar)；(4) 白緣粗吻象鼻蟲 (Graphognathus leucoloma)；(5) 亞洲棕櫚象鼻蟲 (Rhabdoscelus lineaticollis)；(6) 新幾內亞甘蔗象鼻蟲 (R. obscurus) 等六個種類。截至目前，對上述種類之分子鑑定資料尚付之闕如。本計畫之目的在於篩選具有鑑別種間差異之專一性的引子，作為快速鑑定之重要工具。同時，亦將增幅片段定序，建立各種象鼻蟲之DNA資料庫，作為爾後找出種專一性之單點核酸多態型別之基礎，以提高檢測害蟲蟲源與分布之鑑別力。 <br> Abstract: Weevils are insects taxonomically belonging to Coleoptera: Polyphaga: Curculionoidea. One of the most prominent features of these insects is their actively-specialized mandibulate (chewing-type) mouth, which appears long-horned shape due to the fusion with part of the head region. They are categorized as pests because they bite the tissues of roots, fruits and stems, which cause a lot of damages toward crops. Many kinds of weevils laid their eggs on/within crops and their hatched larvae normally live inside the plant tissues. Therefore they can maintain a certain population under the treatment of agrichemicals in most cases and it makes the quarantining process become much more difficult. To date our government have listed six species of weevils with quarantining importance. They are as follows: (1) Acryptorrhychus mangiferae)；(2) A. frigidus； (3) Conotrachelus nenuphar； (4) Graphognathus leucoloma; (5) Rhabdoscelus lineaticollis；(6) R. obscurus。 So far we have not found any reports regarding molecular identification toward the six species listed above. We therefore aim to find species-specific PCR primers to discriminate them, expecting to develop a system of rapid diagnosis. In parallel, we want to select PCR-amplified fragments, establishing species-specific nucleotide databases as the foundation for SNP analysis.象鼻蟲分子鑑定逢機增幅多態型聚合連鎖反應weevilsmolecular identificationRAPDPCR