CHI-KUANG SUNChang, C.-F.C.-F.ChangYu, C.-H.C.-H.YuSun, C.-K.C.-K.SunBrown, C. Tom A.C. Tom A.BrownDeckert, VolkerVolkerDeckertSergeev, Alexer M.Alexer M.SergeevZheltikov, Aleksei M.Aleksei M.ZheltikovCHI-KUANG SUN2018-09-102018-09-102010http://www.scopus.com/inward/record.url?eid=2-s2.0-77955648541&partnerID=MN8TOARShttp://scholars.lib.ntu.edu.tw/handle/123456789/357848Cancer cells require excessive oxygen and nutrition to support their rapid growth, so angiogenesis and decrease of blood oxygen are often associated with areas of cancer development. Current technologies for blood oxygen measurement, however, do not possess high spatial resolution and therefore cannot be used to detect small tumors in their early stage. In this paper, we studied the third harmonic generation (THG) spectra of oxy-and deoxyhemoglobin in the 1170-1365 nm region, which is strongly influenced by the multi-photon resonance effect, especially around the Soret transition band. Our spectroscopic results thus indicate the high potential of THG spectroscopic microscopy for oxygen depletion level measurement of a single red blood cell in vivo. ? 2010 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.Blood or tissue constituent monitoring; Multiharmonic generation; Nonlinear microscopy; Pulse oximetry[SDGs]SDG2[SDGs]SDG3Angiogenesis; Blood oxygen; Cancer cells; Cancer development; Current technology; Deoxyhemoglobin; High potential; High spatial resolution; In-vivo; Multiharmonic generation; Multiphotons; Nonlinear microscopy; Oxygen depletion; Pulse oximetry; Rapid growth; Single red blood cell; Small tumors; Third harmonic generations; Transition bands; Cells; Harmonic generation; Monitoring; Noninvasive medical procedures; Oximeters; Oxygen; Photons; Resonance; Scanning; Blood; deoxyhemoglobin; hemoglobin; oxygen; oxyhemoglobin; absorption; article; blood; human; metabolism; methodology; photon; spectroscopy; Absorption; Hemoglobins; Humans; Oxygen; Oxyhemoglobins; Photons; Spectrum Analysisjournal article10.1002/jbio.201000045