王金和2006-07-262018-07-092006-07-262018-07-092001http://ntur.lib.ntu.edu.tw//handle/246246/28690家禽白血病J 亞群病毒(Subgroup J avian leukosis virus, ALV-J) 引起家禽腫瘤性疾病，造成 產業經濟甚巨。一般皆以ELISA 偵測本病感染的情 形，然而ELISA 無法分辨外源性及內源性病毒的感 染，使其應用的大受限制，本研究的目的即發展出 ELISA 方法分別雞隻受內源性或外源性病毒感 染，供診斷及控制本病之應用。由臺灣三家原種雞 場進行ALV 的檢測及病毒分析，於不同週齡時採 取血液樣本，以酵素免疫吸附法(ELISA) 檢測血 漿中gs Ag (group specific antigen）、抗subgroup J gp85 Ab，再以RT-PCR及核酸定序(sequencing) 確 診亞群感染。結果顯示，於ALV-J 感染雞群早期 ALV Ag 的陽性率上升但非感染場下降;感染雞群 抗ALV-J 抗體上升較早較高。因此在1 週齡及6 週齡檢測雞隻血中gs Ag 的變化情形可以分辨內源 性病毒或ALV-J 感染。此偵測方法的發現對此病的 檢測及控制有莫大的助益。Subgroup J avian leukosis virus (ALV-J) causes serious economic losses in commercial poultry industry. Measuring group-specific (gs) antigen (Ag) by ELISA has been used to identify chickens infected with this virus. However, the inability of ELISA to discriminate the gs Ags from endogenous and exogenous origin has limited its usage. The purpose of the present study was to find a method to discriminate between endogenous and exogenous ALV gs Ag by ELISA. The gs Ag and anti-ALV-J antibody in the plasma samples from chickens at different ages in three grand-parent farms were measured by ELISA. Infected flocks were confirmed by RT-PCR using different subgroup-specific primers. The results indicated that the gs Ag of ALV-J-infected flocks increased, but that of the uninfected flocks decreased in young ages. The anti-ALV-J antibody of infected flocks was higher and increased earlier than uninfected flocks. In conclusion, measuring the gs Ag in blood at the ages of 1 and 6 weeks by ELISA is suitable to discriminate between endogenous and ALV-J infection. This discovery is a valuable method for the detection and control of ALV-J infection.application/pdf62907 bytesapplication/pdfzh-TW國立臺灣大學獸醫學系暨研究所家禽白血病J 亞群病毒群特異抗原反轉錄聚合脢連鎖反應ALV-JELISAgs AntigenRT-PCRreporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/28690/1/892313B002151.pdf