奎諾酮類藥物抗藥性; gyrA基因變異; 高解析熱熔解分析法; campylobacter jejuni; Quinolone resistance; gyrA Mutiation; High-resolution melting assay范揚棋王嘉蘭周崇熙蔡向榮2018-09-102018-09-102014http://www.airitilibrary.com/Publication/Index/16826485-201403-201404240028-201404240028-61-69http://scholars.lib.ntu.edu.tw/handle/123456789/387504A total of 95 isolates of Campylobacter jejuni isolated from domestic waterfowl from 2009 to 2012 in Taiwan were subjected to quinolone resistance analysis. The resistant rates to nalidixic acid and ciprofloxacin were 61.2% (58/95) and 57.9% (55/95), respectively. All isolates were further screened for mutations in the quinolone resistant determining regions (QRDRs) gyrA genes. In the analysis of gyrA mutation, 33.7% of isolates showed no amino acid change, however, 22.1% of isolates were found with one-point mutation at codon 86, 9.5% of isolates at codon 90 and 34.7% of isolates were found with two-point mutations at codon 81 and 86. Four patterns of melting temperature curve were revealed in the high-resolution melting (HRM) analysis. The consistent changes in melting temperatures of different amplicon sequences allow the mutated-gyrA strains to be distinguished from wild-type strains. This rapid detection of gyrA mutations by HRM assay is feasible for extensive quinolone resistance monitoring program.journal article