2011-08-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/664135摘要：相較於電子顯微術、掃描穿隧電子顯微術等顯微技術，原子力顯微術對掃描環境與樣品的限制較小。由於在生化的研究領域中，許多分子特性必須在水溶液環境中量測，因此原子力顯微術在水溶液中的掃描能力備受重視，然而目前要在水溶液環境中取得高解析度影像仍具挑戰性。常用的原子力顯微鏡輕敲模式雖可減小對樣品的破壞，然而微懸臂探針浸入水中後，水的黏滯性不但大幅降低振動的Q值(Quality factor)，甚至阻礙了共振模態的激發。由於微懸臂在介質中受移動阻力，其幾何形狀有著很大的影響。本計畫的研究目的在於創造開發適用於水溶液的微懸臂探針造型，以達到較少介質阻尼的影響，進而提高量測解析度。計畫中我們將以化學蝕刻鎢線方法來製作微探針，再配合其他成型方式完成微懸臂。在儀器設備上則將自行開發原子力顯微鏡控制器與掃描器，以搭配開發的微懸臂探針的性能達到較佳的掃描機制，並整合光學顯微鏡以觀察監測懸臂探針的動態性能。<br> Abstract: In comparision with the electron microscopy and the scanning tunneling microscopy (STM), the atomic force microscopy (AFM) has less limitations on environment and sample material. Because a lot of biomolecules can only live in a liquid environment, an AFM with the scanning capability in liquid is valued for biotechnological research. However, getting high measurement resolution in liquid is still very challenging. The AFM tapping mode induces less damage for sample. Nevertheless, its quality factor (Q-factor) is significantly reduced by the fluid viscosity, so that the the cantilever tip even can’t be resonantly excited. The induced resistant force is significantly related to the geometry of the dipped in fluid medium. The aim of this project is to create and develop cantilever tip adaptable for liquid medium in oder to reduce fluid damping influence and hence improve the measurement resolution. We plan to apply the electrochemical etching process to manufacture the tip from tungsten wire and also utilize the other forming method to make the cantilever. Besides, we plan to develop the controller and the scanner for realizing optimal scanning efficiency for our developed cantilever tip. An optical microscope is also configured to monitor and acquire the dynamic performance.原子力顯微鏡水溶液環境懸臂探針控制器掃描器Atomic force microscopeLiquid environmentCantilever tipControllerScanner