2020-08-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/672054摘要：近年來老化是許多國家所面臨的國安議題，臺灣自1993年即邁入高齡化社會，高齡化速度是歐洲國家和美國的兩倍以上，65歲年長者所佔比例從 1990 年的 6.2% 上升至 2015 年的 12.5%。然而衰老與食慾和食物攝取的下降有關，而其反過來又影響營養狀況，長期影響年長者之健康並造成認知退化及慢性病。 臺灣藜 (Chenopodium formosanum) 稱為穀類的紅寶石，含有多種必需胺基酸、膳食纖維與礦物質，亦含豐富抗氧化及延緩老化功效的酚酸及類黃酮，臺灣藜中如酚類等化合物多以結合態形式存在，且含有部分抗營養物質。以絲狀真菌固態培養產生酵素能水解纖維結構，釋放游離酚進而提升如抗氧化等機能性功效，及降低抗營養因子。發酵時能產生多種酵素，將營養物質小分子化適合高齡者吸收。 本計畫第一年將建立以絲狀真菌固態發酵發芽臺灣藜最適化條件及配方，測定其具有抗衰老功效之酚類及胜&#32957;含量，以及酵素活性、胺基酸組成、一般組成分、質地分析、吸收率與適口性，評估作為台灣藜發酵產品之潛力。計畫第二年將以 Human foreskin fibroblast (HFF) 為細胞模型，測定發酵臺灣藜產品之抗衰老相關研究及可能之機制，計畫第三年延續第二年細胞實驗之成果，以發酵臺灣藜餵食秀麗隱桿線蟲評估延緩老化相關生理功效。 <br> Abstract: Recently, many countries confront national security issues - an aging society. Taiwan has entered an aging society since 1993. The aging rate is more than twice that of European countries and the United States. The proportion of 65-year-olds defined as the elderly&#8207; has increased from 6.2% in 1990. to 12.5% in 2015. However, decreasing appetite and food intake is related to aging, which in turn affects nutritional status, affects the health of the elderly in the long term, and causes cognitive degradation and chronic diseases. Chenopodium formosanum, which called ruby cereal containing a variety of essential amino acids, dietary fiber, minerals, phenolic acids, and flavonoids, is rich in antioxidants and anti-aging effects. Nevertheless, germination C. formosanum exists some anti-nutrients problem, and most nutrition compounds are in a bound state. Enzymes produced by solid-state culture of Rhizopus oligosporus can hydrolyze fibrous structures, release free phenols to enhance useful functions such as antioxidants, and reduce anti-nutritional factors. A variety of enzymes can also be produced by fermentation, which can reduce the molecular weight of nutrients for elderly. In the first year, we will establish the optimal solid-state fermentation conditions and strategy for germination C. formosanum, and determinate total phenols, peptides, enzyme activity, amino acid composition, general composition, texture analysis, absorption rate, and palatability to develop the anti-aging potential fermentation dietary supplements. The second year of the project will use Human foreskin fibroblast (HFF) as a cell model to investigate the anti-aging related research and its possible mechanisms for fermenting germination C. formosanum products. The third year of the plan extend the results of the second year of cell experiments. Use Fermentation dietary supplements to feed Caenorhabditis elegans to evaluate physiological effects related to anti-senescence.臺灣藜銀髮族膳食補充品Chenopodium formosanumelderlydietary supplements