2020-08-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/664646摘要：三維(three-dimensional, 3D)生物列印是近年來興起的一種技術，其結合組織工程與3D列印技術製作各種結構與形狀的生物支架，可應用於修復或重建人體組織和器官。細胞列印墨水材料開發技術門檻高，目前的生物3D列印機台與材料皆各有 缺點，且細胞在列印的過程中亦可能因剪切應力過大導致受損或死亡，因此細胞列印材料與機台的配合為積層製造生物3D列印的關鍵技術。此三年外加十一個月之整合型計畫，在過去三年已完成：(1)可變多噴頭於常溫與體溫下列印不同材料與細胞組合之系統。(2)受損皮膚之3D支架路徑規劃軟體，重建出傷口處原貌。(3)生物體內可降解之含細胞列印材料開發。(4)以裸鼠皮膚受損模型證實，藉由3D列印人工皮膚治療優於傳統治療方法。(5)結合細胞、材料與3D列印技術，以人工皮膚為載具開發具產業化潛力之製程。再接下來的十一個月計畫中，有三個預期完成之主要標的：(1)不規則皮膚傷口支架之曲面層列印製程開發。(2)提升生物墨水之智慧性，使列印的人工皮膚具有更好的仿生性。(3)以3D深度掃描糖尿病大鼠皮膚傷口，並列印掃描之皮膚傷口影像進行傷口修復驗證。透過本計畫之執行，期望能利用所開發之積層細胞列印機台系統與材料，重建受損組織和器官，並進行全套系統的技術授權或新創公司。<br> Abstract: Three-dimensional (3D) bioprinting is an emerging technique to create tissue-like structures for tissue or organ repair and reconstruction. However, the printing system and materials (bioink) commonly utilized for 3D bioprinting have their drawbacks. In addition, the shear stress during the process of printing cell-laden constructs may induce cell damage or cell death. Therefore, the combined printer and material design plays an important role in additive manufacturing for tissue engineering. In the past three years, we have completed: (1) the system of variable multi-nozzle cell-laden printing at room temperature and body temperature; (2) development of the 3D printing path software to reconstruct the original appearance of the wound; (3) biodegradable cell-laden bioinks for 3D printing; (4) skin damage models (nude mouse) used to confirm that the treatment of 3D-printed artificial skin is superior to the traditional treatment; (5) the way to combine cells, bioinks, and 3D printing, and to optimize the printing process of artificial skins with industrialization potential. In the proposed 4th year of the project, we expect to achieve three goals in the next 11 months: (1) to develop the curved layer printing process for irregular skin wound; (2) to improve the intelligent property of the bioink for printing biomimetic cell-laden constructs; (3) to scan the skin wound of diabetic rats with a 3D depth scanner and to print the scanned constructs for clinical wound repair. Through the execution of this project, we expect that the developed 3D bioprinter combined with the new bioink could print the customized constructs to rebuild damaged tissues or organs and the package could be licensed to industry.生物三維列印(細胞列印)生物三維列印機智慧型生物列印材料人工皮膚曲面列印技術。three-dimensional (3D) bioprinting (cell printing)three dimensional (3D) bioprinterintelligent bioinkartificial skincurved layer printing technology.