2019-02-252024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/704501摘要：台灣環境高溫多濕，易使得牛隻產生卵巢早衰，此等繁殖障礙為產業之莫大損失。羊水幹細胞為再生醫學之有用素材，且被證實具有修復卵巢功能之潛力。本研究室先前已成功建立牛羊水幹細胞之培養及其治療應用，經治療之卵巢萎縮牛隻，其發情週期恢復，並可配種成功懷孕產犢。經探討期分子機制之初步結果顯示，microRNA-196 家族成員，包含miR-196a-1、2 及miR-196b 之表現量在幹細胞處理後之卵巢中大幅降低。因此本試驗擬針對microRNA-196 家族之上下游調控之候選因子，分析牛羊水幹細胞造成牛卵巢組織中microRNA-196 家族表現量下降之機制；並分析microRNA-196 家族表現下降後，濾泡形成受修復之機制。若釐清其細節將有助發展以關鍵分子取代幹細胞成為新型治療技術以解決產業困境。<br> Abstract: The climate environment in Taiwan is characterized as hot and humid, which easily causes cows to have premature ovarian failure (POF). POF is a kind of reproductive disorders that makes economic losses to dairy industry. Amniotic fluid-derived stem cells (AFSCs) are useful resources for regenerative medicine, their potential has been proved to restore ovarian function. In our previous study, the cultivation and the therapeutic application of bovine AFSCs (BAFSCs) have been successfully established. After BAFSCs treatment, the estrus cycle could be recovered in those cows used to have bilateral ovarian dystrophy. In addition, the successful pregnancy and calve delivery could be also obtained in those treated cows after artificial insemination. In our preliminary results investigating molecular mechanism, the transcriptional levels of microRNA-196 (miR-196) family, including miR-196a-1, miR-196a-2 and miR-196b showed a significant down-regulation in BAFSCs treated ovary. Therefore, in the present study, we aim to focus on the candidate factors on the up-stream and down-stream regulation to miR-196 family members. The potential mechanism of the down-regulation of miR-196 family by BAFSCs and the recovery regulation of folliculogenesis while miR-196 members were reduced were both determined in this project. If the mechanism could be clarified, it should be helpful to develop a novel therapeutic strategy by replacing stem cells with the critical molecular and finally to solve the reproductive problem in dairy industry.卵巢早衰mir-196 家族牛羊水幹細胞premature ovarian failure (POFmir-196 familybovineamniotic fluid-derived stem cells (AFSCs)