2007-01-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/671353摘要：近年來隨著國際貿易之日趨頻繁，煙草粉蝨隨著人類的活動而提高其擴散範圍，其經濟重要性更為提高。針對形態上不易區分之煙草粉蝨種群，根據其特異性之片段設計專一性之引子來區分生物小種。根據先前所開發之六組煙草粉蝨生物小種專一性引子組(SCAR)為基礎，進行Multiplex PCR於煙草粉蝨生物小種鑑定技術之研發與應用。發展具有高準確性、操作容易、需時短且單次鑑定多個生物小種等之分子鑑定技術，以作為檢防疫之依據與參考。此技術之開發除了可以應用於檢疫防疫上快速鑑定之需求，以及提供農產品貿易談判及避免貿易障礙之科學證據外，亦可應用於田間偵測工作之進行。Multiplex PCR技術之應用將有助於快速鑑定生物小種，針對目標生物小種選擇適合防治方法，更有助於煙草粉蝨不同生物小種防治之參考。<br> Abstract: With the increasing activity of international trades, Bemisia tabaci have emerged to primary pest status with spreading their distributions by human activities. It is generally accepted that morphologically indistinguishable biotypes with different biological traits exist within the B. tabaci species complex. We developed sequence characterized amplified region (SCARs) markers for each of six B. tabaci biotypes. According to SCARs, we will develop Multiplex-PCR and apply this technique to identify biotypes rapidly. The objectives of this survey are to develop rapid identification techniques with highly accuracy, readily application and little running time, to tandardize current methods and protocols, and to produce a summary document to 'nonprofessional' the way to whitefly identification, as a reference source for quarantine treatment, practical information and relevant scientific evidence. Furthermore, we can apply Multiplex-PCR to monitor the dynamics of the B. tabaci in order to prevent its dispersal. Application of Multiplex-PCR will be helpful in rapidly identifying biotypes for quarantine pest control to prevent the invasion of the various biotypes.煙草粉蝨聚合&#37238鏈鎖反應檢疫Multiplex PCRBemisia tabaciQuarantine