環境衛生研究所;Institute of Environmental HealthMA, YEE-CHUNGYEE-CHUNGMALIN, JIA-MINGJIA-MINGLINSUNG, FUNG-CHANGFUNG-CHANGSUNG2008-09-102018-06-302008-09-102018-06-302005http://ntur.lib.ntu.edu.tw//handle/246246/81781Introduction: Carbon dioxide (CO2), volatile organic compounds (VOCs) and environmental tobacco smoke (ETS) are common indoor air pollutants generated from human activities in buildings. This study investigated urinary 8- hydroxydeoxyguanosine (8-OHdG) as an effective biomarker of DNA damage associated with indoor air pollutants among office building occupants. Methods: With consents and indoor CO2 and VOCs measured simultaneously, urinary 8-OHdG and cotinine were determined for 344 non-smokers and 45 smokers recruited from 8 air-conditioned office buildings in Taipei. CO2and total VOCs were measured with portable monitors (Q- TRAK IAQ Model 8551, TSI Incorporated, Shoreview, MN, U.S.A, and PGM-7240, RAE SYSTEMS, California, U.S.A., respectively ). Urinary 8-OHdG and cotinine were determined by enzyme- linked immunosorbent assay (ELISA) and barbituric acid assay , respectively. Results: There were large variations in concentrations of CO2 (median= 901 ppm; range=“530~2794” ppm) and total VOCs (median= 190 ppb; range= 1~55733 ppb), and urinary cotinine levels (median= 2135 mg/g creatinine; range= 486~8984 mg/g creatinine). The average 8-OHdG levels were approximately two times higher in smokers than in non- smokers for both females (5.06 vs. 11.3 mg/g creatinine) and males (5.08 vs.10.6 mg/g creatinine). The multivariate logistic regression analysis showed that, comparing with individuals with CO2 exposure at the lowest quartile, the odds ratios (ORs) of having the urinary 8-OHdG levels greater than the overall median value, 4.99 mg/g creatinine, were 7.66 (95% confidence interval (CI) = 3.08–19.1) for individuals with the exposure at the third quartile level, and 14.3 (95% CI = 14.13–49.7) for those at the highest quartile level. Smokers were 6.33 times (95% CI = 1.44–27.8 ) more likely than never smokers to have such an elevated urinary 8-OHdG level. There was no significant association between VOCs and 8-OHdG excreted. Conclusion: The indoor CO2 and tobacco smoke exposure contribute independently to elevated DNA damage, using urinary 8-OHdG levels as a sensitive biomarker.en-USjournal article